Back to Search Start Over

Crocetin protects against oxidative damage in rat primary hepatocytes

Authors :
Tsui-Hwa Tseng
Chau-Jong Wang
Jin-Ming Huang
Song-Jui Shiow
Chia-Yih Chu
Source :
Cancer Letters. 97:61-67
Publication Year :
1995
Publisher :
Elsevier BV, 1995.

Abstract

Crocetin is a major component in the fruit of Gardenia jaminoides Ellis, a Chinese herbal medicine. Its protective action and mechanism against oxidative damage were investigated and mechanism against oxidative damage were investigated. Reactive oxygen species (ROS) were generated enzymatically in the xanthine-xanthine oxidase (X/XO 5 microM/0.01 u/ml) system and non-enzymatically in the paraquat (PQ 5 mM) system. Both systems increased leakage of lactate dehydrogenase (LDH) and alanine transaminase (ALT) in rat primary hepatocytes, but the hepatotoxicity was significantly suppressed on pretreatment with crocetin (10, 20 microM). Crocetin decreased formation of malondialdehyde (MDA) as an index of lipid peroxidation induced by ROS. The oxyradical generation by X/XO or PQ caused DNA damage evaluated with unscheduled DNA synthesis (UDS) in rat primary hepatocytes. The addition of crocetin decreased genotoxicity evaluated with UDS in both systems. The data showed that crocetin also inhibited the formation of superoxide anion in the X/XO system and bleached the free radical 1, 1-diphenyl-2- picrylhydrazyl (DPPH). The protective action of crocetin operated via quenching of the superoxide anion and/or free radical.

Details

ISSN :
03043835
Volume :
97
Database :
OpenAIRE
Journal :
Cancer Letters
Accession number :
edsair.doi.dedup.....959967f269b891f42229100cf68818de
Full Text :
https://doi.org/10.1016/0304-3835(95)03964-x