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Barley produced Culicoides allergens are suitable for monitoring the immune response of horses immunized with E. coli expressed allergens
- Source :
- Veterinary Immunology and Immunopathology. 201:32-37
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Insect bite hypersensitivity is an allergic dermatitis of horses caused by bites of Culicoides midges. Sufficient amount of pure, endotoxin-free allergens is a prerequisite for development and monitoring of preventive and therapeutic allergen immunotherapy. Aims of the study were to compare the Culicoides nubeculosus (Cul n) allergens Cul n 3 and Cul n 4, produced in transgenic barley grains with the corresponding E. coli or insect cells expressed proteins for measuring antibody and cytokine responses. Allergen-specific IgG responses were measured by ELISA in sera from twelve horses not exposed to Culicoides, before and after vaccination with E. coli-rCul n 3 and 4. Before vaccination no IgG binding to the barley and insect cell produced proteins was detected and a similar increase in specific IgG was observed after vaccination. While IgG levels to the E.coli expressed proteins were higher in the post-vaccination sera, some background binding was observed pre-vaccination. In vitro re-stimulation of PBMC was performed for measurements of cytokines. E. coli expressed proteins resulted in high background in PBMC from non-vaccinated controls. The barley and insect cell expressed proteins induced similar amount of IFN-γ and IL-4 in PBMC from vaccinated horses. Barley produced allergens are promising tools for use in immunoassays.
- Subjects :
- 0301 basic medicine
Allergy
040301 veterinary sciences
medicine.medical_treatment
Immunology
Enzyme-Linked Immunosorbent Assay
Ceratopogonidae
Microbiology
Serology
0403 veterinary science
03 medical and health sciences
Blood serum
Immune system
Escherichia coli
Hypersensitivity
medicine
Animals
Horses
Cloning, Molecular
General Veterinary
biology
Insect Bites and Stings
Hordeum
04 agricultural and veterinary sciences
Immunotherapy
Allergens
Plants, Genetically Modified
Culicoides
biology.organism_classification
medicine.disease
030104 developmental biology
Desensitization, Immunologic
IgG binding
Immunoglobulin G
Leukocytes, Mononuclear
biology.protein
Cytokines
Insect Proteins
Horse Diseases
Immunization
Antibody
Subjects
Details
- ISSN :
- 01652427
- Volume :
- 201
- Database :
- OpenAIRE
- Journal :
- Veterinary Immunology and Immunopathology
- Accession number :
- edsair.doi.dedup.....957a4bcc85ef7d6b84f3459bab302852