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Fingerprinting of Flavobacterium psychrophilum isolates by ribotyping and plasmid profiling
- Source :
- Diseases of Aquatic Organisms. 33:167-177
- Publication Year :
- 1998
- Publisher :
- Inter-Research Science Center, 1998.
-
Abstract
- Flavobacterium psychrophilum is the agent of cold-water disease and rainbow trout fry syndrome in salmonid fish worldwide. Ribosomal RNA gene restriction patterns (ribotypes) and plasmid profiles were determined on a collection of 85 strains isolated from different countries and fish species. Several ribotypes were obtained by using the restriction endonucleases Hinc II and Pvu II. Computer analysis of the ribotypes revealed that some of them were clearly associated with the fish species from which the strains were isolated, whereas no correlation with the geographical origin was found. Most of the strains harboured at least one plasmid and several different plasmid profiles were observed, even among strains sharing the same ribotype. These methods, used alone or in combination with other typing techniques, can be considered powerful tools for the epidemiological tracing of F. psychrophilum infections.
- Subjects :
- DNA, Bacterial
Restriction Mapping
Flavobacterium psychrophilum
Aquatic Science
DNA, Ribosomal
Flavobacterium
Bacterial cold water disease
Microbiology
Restriction fragment
Fish Diseases
03 medical and health sciences
Ribotyping
Plasmid
Image Processing, Computer-Assisted
Animals
Cluster Analysis
14. Life underwater
Typing
Deoxyribonucleases, Type II Site-Specific
Ecology, Evolution, Behavior and Systematics
030304 developmental biology
Genetics
0303 health sciences
biology
030306 microbiology
Ribosomal RNA
DNA Fingerprinting
Restriction enzyme
RNA, Ribosomal
biology.protein
Gram-Negative Bacterial Infections
Salmonidae
Densitometry
Plasmids
Subjects
Details
- ISSN :
- 16161580 and 01775103
- Volume :
- 33
- Database :
- OpenAIRE
- Journal :
- Diseases of Aquatic Organisms
- Accession number :
- edsair.doi.dedup.....9554c2bfc45837c5df97269721dc0d4a
- Full Text :
- https://doi.org/10.3354/dao033167