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Fingerprinting of Flavobacterium psychrophilum isolates by ribotyping and plasmid profiling

Authors :
Maria C. Urdaci
Jean-François Bernardet
Francine Grimont
Chehid Chakroun
Source :
Diseases of Aquatic Organisms. 33:167-177
Publication Year :
1998
Publisher :
Inter-Research Science Center, 1998.

Abstract

Flavobacterium psychrophilum is the agent of cold-water disease and rainbow trout fry syndrome in salmonid fish worldwide. Ribosomal RNA gene restriction patterns (ribotypes) and plasmid profiles were determined on a collection of 85 strains isolated from different countries and fish species. Several ribotypes were obtained by using the restriction endonucleases Hinc II and Pvu II. Computer analysis of the ribotypes revealed that some of them were clearly associated with the fish species from which the strains were isolated, whereas no correlation with the geographical origin was found. Most of the strains harboured at least one plasmid and several different plasmid profiles were observed, even among strains sharing the same ribotype. These methods, used alone or in combination with other typing techniques, can be considered powerful tools for the epidemiological tracing of F. psychrophilum infections.

Details

ISSN :
16161580 and 01775103
Volume :
33
Database :
OpenAIRE
Journal :
Diseases of Aquatic Organisms
Accession number :
edsair.doi.dedup.....9554c2bfc45837c5df97269721dc0d4a
Full Text :
https://doi.org/10.3354/dao033167