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Analysis of codherin/catenin complexes in transformed thyroid epithelial cells: Modulation by beta 1 integrin subunit
- Source :
- European journal of cell biology, 79 (2000): 583–593. doi:10.1078/0171-9335-00083, info:cnr-pdr/source/autori:Celetti, A; Garbi, C; Consales, C; Cerrato, A; Greco, D; Mele, E; Nitsch, L; Grieco, M/titolo:Analysis of codherin%2Fcatenin complexes in transformed thyroid epithelial cells: Modulation by beta 1 integrin subunit/doi:10.1078%2F0171-9335-00083/rivista:European journal of cell biology (Print)/anno:2000/pagina_da:583/pagina_a:593/intervallo_pagine:583–593/volume:79, Scopus-Elsevier
- Publication Year :
- 2000
- Publisher :
- Elsevier, Urban u. Fischer, München , Germania, 2000.
-
Abstract
- We have analysed the expression of cadherin/catenin complex molecules in PC C13 rat thyroid cells transformed in vitro with different oncogenes, No significant downregulation of either E-cadherin, alpha-, beta- and gamma-catenin was detected following the introduction of activated forms of myc, adenovirus E1A, ras, raf, myc + ras, EIA + raf, However, ras- and raf-transformed PC C13 cells showed altered adherens junctions. An altered distribution of cadherin/catenin complexes characterized by radially oriented membrane spikes perpendicular to cell edges was the most prominent feature evidenced by immunofluorescence. No beta 1 integrin localization was observed in areas where this altered pattern of E-cadherin expression was detected. However, beta 1 integrin submit expression was detected at areas of cell-cell contact where E-cadherin showed a normal pattern of expression. Furthermore, ras- and raf-transformed PC C13 cells showed the ability to migrate in collagen gels, in contrast to their normal untransformed counterpart, Overexpression of beta 1 integrin was found to restore normal E-cadherin localization at cell-cell contacts and to partially inhibit the ability to migrate in collagen gels. Finally, two cell lines obtained by ras transformation in vivo, and derived from a rat primary thyroid carcinoma (TK6) and its lung metastasis (MPTK6), were found to have lost gamma-catenin expression. TK6 lost also E-cadherin expression and membrane localization of alpha-catenin, These results suggest that: i) in vitro thyroid cell transformation is associated to a change in cadherin/catenin complexes distribution rather than to a decrease in expression; ii) in vivo transformation is associated to the loss of expression of some of these molecules likely due to tumor progression; iii) alterations in beta 1 integrin subunit expression can result in changes in cadherin/catenin function thus implying that an integrin-cadherin synergy may exist in thyroid cells.
- Subjects :
- Histology
Beta-catenin
integrin
Beta I integrin
Blotting, Western
Retroviridae Proteins, Oncogenic
Genes, myc
Thyroid Gland
Alpha catenin
Fluorescent Antibody Technique
Gene Expression
Cell Communication
Oncogene Proteins v-raf
Pathology and Forensic Medicine
thyroid
Sarcoma Viruses, Murine
Cell Movement
Animals
beta Catenin
Cell Line, Transformed
Thyroid Epithelial Cells
biology
Cadherin
Integrin beta1
Epithelial Cells
Cell Biology
General Medicine
Cadherins
Molecular biology
catenins
Rats
Cell biology
Catenin
Cytoskeletal Proteins
Genes, ras
cadherin
Desmoplakins
Integrin alpha M
Trans-Activators
biology.protein
beta 1 integrin
Integrin, beta 6
Adenovirus E1A Proteins
Collagen
gamma Catenin
Catenin complex
Gels
alpha Catenin
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- European journal of cell biology, 79 (2000): 583–593. doi:10.1078/0171-9335-00083, info:cnr-pdr/source/autori:Celetti, A; Garbi, C; Consales, C; Cerrato, A; Greco, D; Mele, E; Nitsch, L; Grieco, M/titolo:Analysis of codherin%2Fcatenin complexes in transformed thyroid epithelial cells: Modulation by beta 1 integrin subunit/doi:10.1078%2F0171-9335-00083/rivista:European journal of cell biology (Print)/anno:2000/pagina_da:583/pagina_a:593/intervallo_pagine:583–593/volume:79, Scopus-Elsevier
- Accession number :
- edsair.doi.dedup.....953785cd153bd4a6049d6742db34a40d