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Consecutive influenza surveillance of neuraminidase mutations and neuraminidase inhibitor resistance in Japan
- Source :
- Influenza and Other Respiratory Viruses
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- Background The large consumption of neuraminidase inhibitors (NAIs) for the treatment of influenza virus infections places Japan at risk of becoming the epicenter of the global spread of NAI-resistant viruses. Objective To clarify NA amino acid mutations of epidemic influenza viruses in Japan and their related NAI resistance. Methods A total of 1791 samples, including 396 A/H1N1pdm09, 1117 A/H3N2, and 278 B isolates, were collected to determine of their 50% inhibitory concentration (IC50 ) values by NAIs (oseltamivir, zanamivir, peramivir, and laninamivir) during the Japanese seasons from 2011-2012 to 2016-2017. Then, 380 samples including 49 A/H1N1pdm09, 251 A/H3N2, and 80 B isolates were sequenced for the entire NA genes. Results Neuraminidase inhibitor-resistant A/H1N1pdm09 viruses were detected at a frequency of 1.3% (5/396 isolates) in the epidemic seasons. None of the A/H3N2 and B viruses developed resistance to any of the four NAIs during the six seasons. Only five and 13 AA mutations were detected in the NA catalytic sites of A/H1N1pdm09 and A/H3N2 viruses, respectively. No mutations were observed in the catalytic sites of B viruses. Four of the five mutations in the catalytic sites of A/H1N1pdm09 consisted of H275Y, which was related to high resistance to oseltamivir and peramivir. Most (10/13) of the catalytic site mutations in A/H3N2 were associated with MDCK-passaged induction (D151G/N). Finally, no mutations related to substantial NAI resistance were detected in the A/H3N2 and B viruses examined. Conclusion These findings suggest that the NA catalytic sites of influenza viruses are well preserved. Even in Japan, no spread of NAI-resistant viruses has been observed, and A/H1N1pdm09 viruses carrying H275Y remain limited.
- Subjects :
- Pulmonary and Respiratory Medicine
Oseltamivir
Epidemiology
medicine.drug_class
viruses
Neuraminidase
medicine.disease_cause
Antiviral Agents
Virus
resistance
Inhibitory Concentration 50
chemistry.chemical_compound
Influenza A Virus, H1N1 Subtype
Zanamivir
Japan
Drug Resistance, Viral
Influenza, Human
medicine
Humans
Enzyme Inhibitors
Mutation
biology
Neuraminidase inhibitor
neuraminidase inhibitor
Public Health, Environmental and Occupational Health
virus diseases
Original Articles
Laninamivir
Virology
Infectious Diseases
chemistry
Epidemiological Monitoring
biology.protein
Original Article
Peramivir
influenza
medicine.drug
Subjects
Details
- ISSN :
- 17502659 and 17502640
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Influenza and Other Respiratory Viruses
- Accession number :
- edsair.doi.dedup.....9521b98a0be04677af7b4415b526cb64