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Urine is superior to saliva when screening for postnatal CMV infections in preterm infants
- Source :
- Journal of Clinical Virology. 61:61-64
- Publication Year :
- 2014
- Publisher :
- Elsevier BV, 2014.
-
Abstract
- Background Cytomegalovirus (CMV) is the most frequently contracted virus in preterm infants. Postnatal infection is mostly asymptomatic but is sometimes associated with severe disease. To diagnose an infection, urine or saliva samples can be tested for CMV-DNA by real-time polymerase chain reaction (rtPCR). Although the diagnostic accuracy of testing saliva samples has not been determined in preterm infants, saliva is widely used because it is easier to obtain than urine. Objectives To determine whether screening of saliva is equivalent to urine to detect a postnatal CMV infection in preterm infants. Study design Between 2010 and 2013 saliva and urine samples were collected from infants admitted to the Neonatal Intensive Care Unit of the University Medical Center Utrecht and born with a gestational age (GA) below 32 weeks. Urine samples were obtained within three weeks after birth and urine and saliva samples at term equivalent age (40 weeks GA) and tested for CMV-DNA by rtPCR. Infants with a congenital CMV infection were excluded. Results Of 261 preterm infants included in the study, CMV-DNA was detected in urine of 47 and in saliva of 43 children. Of 47 infants with postnatal CMV infection, CMV was detected in 42 saliva samples (sensitivity 89.4%; CI 76.9–96.5). Of 214 children without postnatal CMV infection, one saliva sample tested positive for CMV (specificity 99.5%; CI 97.4–99.9). Conclusions Screening saliva for CMV-DNA by rtPCR is inferior to urine to diagnose postnatal CMV infections in preterm infants.
- Subjects :
- medicine.medical_specialty
Saliva
Neonatal intensive care unit
Cmv infections
Congenital cytomegalovirus infection
Cytomegalovirus
Urine
Real-Time Polymerase Chain Reaction
Sensitivity and Specificity
Asymptomatic
Gastroenterology
Virology
Internal medicine
medicine
Humans
business.industry
Infant, Newborn
Infant
virus diseases
Gestational age
medicine.disease
Infectious Diseases
Real-time polymerase chain reaction
Cytomegalovirus Infections
DNA, Viral
Immunology
medicine.symptom
business
Infant, Premature
Subjects
Details
- ISSN :
- 13866532
- Volume :
- 61
- Database :
- OpenAIRE
- Journal :
- Journal of Clinical Virology
- Accession number :
- edsair.doi.dedup.....951eb2e7a2262c2688269091cad2addd