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Modeling Spontaneous Metastatic Renal Cell Carcinoma (mRCC) in Mice Following Nephrectomy
- Source :
- Journal of Visualized Experiments.
- Publication Year :
- 2014
- Publisher :
- MyJove Corporation, 2014.
-
Abstract
- One of the key challenges to improved testing of new experimental therapeutics in renal cell carcinoma (RCC) is the development of models that faithfully recapitulate early- and late-stage metastatic disease progression. Typical tumor implantation models utilize ectopic or orthotopic primary tumor implantation, but few include systemic spontaneous metastatic disease that mimics the clinical setting. This protocol describes the key steps to develop RCC disease progression stages similar to patients. First, it uses a highly metastatic mouse tumor cell line in a syngeneic model to show orthotopic tumor cell implantation. Methods include superficial and internal implantation into the sub-capsular space with cells combined with matrigel to prevent leakage and early spread. Next it describes the procedures for excision of tumor-bearing kidney (nephrectomy), with critical pre- and post- surgical mouse care. Finally, it outlines the steps necessary to monitor and assess micro-and macro-metastatic disease progression, including bioluminescent imaging as well provides a detailed visual necropsy guide to score systemic disease distribution. The goal of this protocol description is to facilitate the widespread use of clinically relevant metastatic RCC models to improve the predictive value of future therapeutic testing.
- Subjects :
- Oncology
Systemic disease
medicine.medical_specialty
Pathology
medicine.medical_treatment
General Chemical Engineering
Cell
Disease
Kidney
Nephrectomy
General Biochemistry, Genetics and Molecular Biology
Mice
Renal cell carcinoma
Internal medicine
medicine
Animals
Humans
Neoplasm Metastasis
Carcinoma, Renal Cell
Matrigel
General Immunology and Microbiology
business.industry
General Neuroscience
medicine.disease
Primary tumor
Kidney Neoplasms
medicine.anatomical_structure
Luminescent Measurements
Disease Progression
Medicine
business
Neoplasm Transplantation
Subjects
Details
- ISSN :
- 1940087X
- Database :
- OpenAIRE
- Journal :
- Journal of Visualized Experiments
- Accession number :
- edsair.doi.dedup.....950fb3d7b2be8aed6b7435f4856e416d