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Differential inhibition of macrophage microbicidal activity by liposomes
- Source :
- Infection and immunity. 47(2)
- Publication Year :
- 1985
-
Abstract
- In vitro culture of murine resident peritoneal macrophages with lymphokine (LK)-rich leukocyte culture fluids induces enhanced microbicidal activity against amastigotes of the protozoan parasite Leishmania tropica. Macrophages infected with Leishmania and treated with LKs after infection acquire the capacity to kill the intracellular parasite within 72 h. When compared with control macrophage cultures treated with medium lacking LKs, 80 to 90% fewer macrophages treated with LKs contained amastigotes. In experiments designed to test liposome delivery of LKs to infected macrophages, addition of multilamellar liposomes composed of phosphatidylcholine and phosphatidylserine (molar ratio, 7:3) completely abrogated LK-induced microbicidal activity. Liposomes containing only phosphatidylcholine were not inhibitory. Inhibition of LK activity by the liposomes occurred regardless of whether the liposomes contained LKs. Liposomal inhibition of activated macrophage effector activity was limited to intracellular killing; LK-induced macrophage extracellular cytolysis (i.e., tumor cytotoxicity) was not affected by liposome treatment. These data indicate that elucidation of the effects of liposome composition on acquired host defense mechanisms may be useful for the design of drug delivery systems that allow expression or augmentation of immunologically induced mechanisms for the intracellular destruction of infectious agents.
- Subjects :
- Immunology
Phosphatidylserines
Biology
Microbiology
chemistry.chemical_compound
Mice
Macrophage
Animals
Leishmania
Liposome
Lymphokines
Intracellular parasite
Macrophages
Lymphokine
Phosphatidylserine
Macrophage Activation
biology.organism_classification
Cytolysis
Infectious Diseases
chemistry
Depression, Chemical
Liposomes
Phosphatidylcholines
Parasitology
Intracellular
Research Article
Subjects
Details
- ISSN :
- 00199567
- Volume :
- 47
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Infection and immunity
- Accession number :
- edsair.doi.dedup.....95027052f427222665433a699e068bc1