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Human papillomavirus type 16 (HPV 16) E7 and major histocompatibility complex (MHC) class I and II expression in human keratinocytes in culture
- Source :
- Archives of Virology. 141:449-458
- Publication Year :
- 1996
- Publisher :
- Springer Science and Business Media LLC, 1996.
-
Abstract
- The low expression of major histocompatibility complex (MHC) class I antigens on human papillomavirus (HPV)-infected cervical carcinoma cells may be responsible for an insufficient cytotoxic T cell response against these cells. To investigate in vitro whether the HPV type 16 early gene product E7 influences cell surface expression of MHC class I and II molecules the HPV negative keratinocyte cell line HaCaT was either stably transfected with the E7 gene or infected with E7-recombinant vaccinia viruses. No difference in MHC class I transcription was detected between E7-transfected and untransfected HaCaT cells. MHC class I cell surface expression as determined by FACS analysis was stronger in some of the transfectants and less intensive in others when compared to untransfected HaCaT cells. In wildtype as well as in E7-recombinant vaccinia virus infected HaCaT cells downregulation of MHC class I molecules on protein and transcriptional level was observed. The alterations in MHC class I expression were independent of the presence and amount of E7-specific transcripts. None of the transfectants or infected HaCaT cells had MHC class II molecules on their cell surface. Hence, our data did not show a correlation between HPV 16 E7 and MHC expression in vitro.
- Subjects :
- Gene Expression Regulation, Viral
Keratinocytes
Papillomavirus E7 Proteins
viruses
Genes, MHC Class II
CD1
Gene Expression
Genes, MHC Class I
Vaccinia virus
Transfection
Major histocompatibility complex
Cell Line
Virology
MHC class I
Tumor Cells, Cultured
Humans
Cytotoxic T cell
Papillomaviridae
MHC class II
biology
Oncogene Proteins, Viral
General Medicine
MHC restriction
HaCaT
biology.protein
CD8
Subjects
Details
- ISSN :
- 14328798 and 03048608
- Volume :
- 141
- Database :
- OpenAIRE
- Journal :
- Archives of Virology
- Accession number :
- edsair.doi.dedup.....94f479e431d4eb511dc9c1d30f150e55