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A possible restriction of ferro- and ferricyanide oxidoreductase activities of rat liver mitochondria by the outer membrane
- Source :
- Archives of biochemistry and biophysics. 443(1-2)
- Publication Year :
- 2005
-
Abstract
- In this work, various ferro-ferricyanide oxidoreductase activities of rat liver mitochondria were studied to find conditions under which the outer membrane might restrict the flux of these highly charged non-biological anions. When the isotonic low ionic strength medium was supplemented with 25 mM KCl, a several-fold increase in the succinate-ferricyanide reductase activity of mitochondria and in the rate of external NADH oxidation in the presence of ferrocyanide was observed. Mitochondrial respiration with 5 mM ferrocyanide was almost completely inhibited after consumption of 3.8–18.5% of the dissolved oxygen, depending on the medium and the presence of 2,4-dinitrophenol. These and other experimental data together with mathematical modeling of the redox-state equilibrium suggest that the measured activities might be restricted by two factors: first, the permeability of the outer mitochondrial membrane and second, a strong influence of the ionic strength of incubation media on the intermembrane space redox reactions.
- Subjects :
- Male
Biophysics
Mitochondria, Liver
Mitochondrion
Biochemistry
Models, Biological
chemistry.chemical_compound
Oxidoreductase
Animals
NADH, NADPH Oxidoreductases
Inner mitochondrial membrane
Ferricyanides
Molecular Biology
Cells, Cultured
chemistry.chemical_classification
Rats
Enzyme Activation
chemistry
Ionic strength
Mitochondrial Membranes
Ferricyanide
Ferrocyanide
Bacterial outer membrane
Intermembrane space
Oxidoreductases
Ferrocyanides
Subjects
Details
- ISSN :
- 00039861
- Volume :
- 443
- Issue :
- 1-2
- Database :
- OpenAIRE
- Journal :
- Archives of biochemistry and biophysics
- Accession number :
- edsair.doi.dedup.....94e8f0f74bb094938aa3437bfef3a753