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Cellular uptake of plain and SPION-modified microbubbles for potential use in molecular imaging
- Source :
- Cellular and Molecular Bioengineering
- Publication Year :
- 2017
- Publisher :
- Springer, 2017.
-
Abstract
- Introduction Both diagnostic ultrasound (US) and magnetic resonance imaging (MRI) accuracy can be improved by using contrast enhancement. For US gas-filled microbubbles (MBs) or silica nanoparticles (SiNPs), and for MRI superparamagnetic or paramagnetic agents, contribute to this. However, interactions of MBs with the vascular wall and cells are not fully known for all contrast media. Methods We studied the in vitro interactions between three types of non-targeted air-filled MBs with a polyvinyl-alcohol shell and murine macrophages or endothelial cells. The three MB types were plain MBs and two types that were labelled (internally and externally) with superparamagnetic iron oxide nanoparticles (SPIONs) for US/MRI bimodality. Cells were incubated with MBs and imaged by microscopy to evaluate uptake and adhesion. Interactions were quantified and the MB internalization was confirmed by fluorescence quenching of non-internalized MBs. Results Macrophages internalized each MB type within different time frames: plain MBs 6 h, externally labelled MBs 25 min and internally labelled MBs 2 h. An average of 0.14 externally labelled MBs per cell were internalized after 30 min and 1.34 after 2 h; which was 113% more MBs than the number of internalized internally labelled MBs. The macrophages engulfed these three differently modified new MBs at various rate, whereas endothelial cells did not engulf MBs. Conclusions Polyvinyl-alcohol MBs are not taken up by endothelial cells. The MB uptake by macrophages is promoted by SPION labelling, in particular external such, which may be important for macrophage targeting.
- Subjects :
- medicine.medical_specialty
animal structures
Diagnostic ultrasound
Superparamagnetic iron oxide nanoparticles
Macrophage targeting
media_common.quotation_subject
education
02 engineering and technology
030204 cardiovascular system & hematology
polyvinyl-alcoho
macrophages
endothelial cells
interactions
in vitro
General Biochemistry, Genetics and Molecular Biology
Article
03 medical and health sciences
0302 clinical medicine
Polyvinyl-alcohol
medicine
Internalization
media_common
Settore CHIM/02 - Chimica Fisica
Chemistry
Adhesion
021001 nanoscience & nanotechnology
In vitro
Modeling and Simulation
Microbubbles
Biophysics
Radiology
Molecular imaging
0210 nano-technology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Cellular and Molecular Bioengineering
- Accession number :
- edsair.doi.dedup.....94e17e7779b8a883c910bb9b643eded9