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Isolation and expansion of synovial CD34(-)CD44(+)CD90(+) mesenchymal stem cells: comparison of an enzymatic method and a direct explant technique
- Source :
- Connective tissue research. 52(3)
- Publication Year :
- 2010
-
Abstract
- Synovium-derived mesenchymal stem cells (MSCs) offer a promising therapeutic option for cartilage regeneration. The conventional method of MSC isolation involves single-cell suspensions using collagenases. Recently, a nonenzymatic explant technique was developed to isolate MSCs. We compared these techniques in the isolation of functional MSCs. MSCs were isolated from human fibrous and adipose synovium of osteoarthritic patients using explants or enzymatic methods. Total cell number, percentage of MSCs, and surface marker expression of MSCs were measured following expansion. Multipotentiality was determined using a MSC functional identification kit. MSCs isolated from fibrous or adipose synovium using these two techniques expressed similar levels of the surface markers CD44, CD90, and CD105, and displayed similar multipotentiality in generating adipocytes, osteoblasts, and chondrocytes. Total cell number and number of CD34(-)CD44(+)CD90(+) MSCs after 10-day expansion were similar in each culture, regardless of the source and method used, although the percentage of MSCs was slightly higher in explant cultures. There were no correlations between MSC yield and patient age, Hospital for Special Surgery score, and degree of deformity under all culture conditions. Both the enzymatic and explant techniques yielded similar yields of MSCs with similar characteristics. Because the explant technique is simpler and less invasive, it may be preferred over enzymatic techniques for isolating MSCs from the synovium of osteoarthritic patients for cartilage regeneration.
- Subjects :
- Male
CD34
Cell Culture Techniques
Adipose tissue
Antigens, CD34
Cell Separation
Biochemistry
Rheumatology
Antigens, CD
Synovial Fluid
medicine
Humans
Orthopedics and Sports Medicine
CD90
Collagenases
Molecular Biology
Aged
Cell Proliferation
Demography
Aged, 80 and over
Chemistry
Regeneration (biology)
Cartilage
Multipotent Stem Cells
Mesenchymal stem cell
Mesenchymal Stem Cells
Cell Biology
Middle Aged
Cell biology
medicine.anatomical_structure
Hyaluronan Receptors
Adipose Tissue
Immunology
Thy-1 Antigens
Female
Explant culture
Multipotentiality
Subjects
Details
- ISSN :
- 16078438
- Volume :
- 52
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Connective tissue research
- Accession number :
- edsair.doi.dedup.....94cef82b0ada904c87444f8ce1e76feb