Optimization of Fenton process on removing antibiotic resistance genes from excess sludge by single-factor experiment and response surface methodology

Excess sludge contains large amounts of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs), posing a risk for human health. However, most current studies usually ignored their abundance and removal in excess sludge. Therefore, this study aimed to reduce ARGs/MGEs in sludge by Fenton process, and applied single-factor experiment (SFE) and response surface methodology (RSM) to optimize the Fenton reaction condition for higher removal rates of ARGs/MGEs. The results demonstrated that the removal rates of target genes by SFE optimized condition ranged from 10.91% to 66.86%, while the removal rates caused by RSM optimized condition were 48.02% - 76.36%, indicating RSM was a useful tool to improve the removal rates of ARGs in excess sludge. Additionally, the scanning electron microscope and cell apoptosis results suggested that the Fenton treatment altered the structure of sludge and reduced the numbers of normal cells, thus causing the reductions of target genes.