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Multispectral imaging for highly accurate analysis of tumour-infiltrating lymphocytes in primary melanoma
- Source :
- Histopathology, 70, 4, pp. 643-649, Histopathology, 70, 643-649, Histopathology, 70(4), 643. Wiley-Blackwell
- Publication Year :
- 2016
-
Abstract
- Contains fulltext : 173035.pdf (Publisher’s version ) (Open Access) AIMS: The quality and quantity of the infiltration of immune cells into tumour tissues have substantial impacts on patients' clinical outcomes, and are associated with response to immunotherapy. Therefore, the precise analysis of tumour-infiltrating lymphocytes (TILs) is becoming an important additional pathological biomarker. Analysis of TILs is usually performed semiquantitatively by pathologists on haematoxylin and eosin-stained or immunostained tissue sections. However, automated quantification outperforms semiquantitative approaches, and is becoming the standard. Owing to the presence of melanin pigment, this approach is seriously hampered in melanoma, because the spectrum of melanin lies close to that of commonly used immunohistochemical stains. Aim of this study is to overcome the technical issues due to the presence of melanin for an automated and accurate quantification of TILs in melanoma. METHODS AND RESULTS: Here, we successfully applied a novel multispectral imaging (MSI) technique to enumerate T cells in human primary melanomas. This microscopy technique combines imaging with spectroscopy to obtain both quantitative expression data and the tissue distributions of different cellular markers. We demonstrate that MSI allows complete and accurate analysis of TILs, successfully avoiding the blurring of images by melanin pigments, in whole tissue slide primary melanoma lesions, which could otherwise not be accurately detected by conventional digital image methodologies. CONCLUSIONS: Our study highlights the potential of MSI for accurate assessment of immune cell infiltrates, including those in notoriously difficult tissues, such as pigmented melanomas. Quantification of tumour infiltration by different immune cell types is crucial in the search for new biomarkers to predict patient responses to immunotherapies. Our findings show that this innovative microscopy technique is an important extension of the armamentarium of pathologists.
- Subjects :
- 0301 basic medicine
Diagnostic Imaging
Cell type
Pathology
medicine.medical_specialty
Histology
Melanoma/immunology
Skin Neoplasms
medicine.medical_treatment
Cancer development and immune defence Radboud Institute for Molecular Life Sciences [Radboudumc 2]
Skin Neoplasms/immunology
Biology
Rare cancers Radboud Institute for Molecular Life Sciences [Radboudumc 9]
Pathology and Forensic Medicine
Melanin
Lymphocytes, Tumor-Infiltrating/pathology
03 medical and health sciences
Diagnostic Imaging/methods
0302 clinical medicine
Lymphocytes, Tumor-Infiltrating
Image Interpretation, Computer-Assisted
medicine
Computer-Assisted/methods
Journal Article
Humans
Lymphocytes
Image Interpretation
Melanoma
Microscopy
Spectrum Analysis
Image Interpretation, Computer-Assisted/methods
Digital pathology
General Medicine
Immunotherapy
medicine.disease
3. Good health
030104 developmental biology
030220 oncology & carcinogenesis
Biomarker (medicine)
Immunohistochemistry
Tumor-Infiltrating/pathology
Infiltration (medical)
Spectrum Analysis/methods
Microscopy/methods
Algorithms
Subjects
Details
- ISSN :
- 13652559 and 03090167
- Volume :
- 70
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Histopathology
- Accession number :
- edsair.doi.dedup.....94bc909efdce00ed04d9fed08c672ac9