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Method for the Production and Purification of Plant Immuno-Active Xylanase from Trichoderma
- Source :
- International Journal of Molecular Sciences, Vol 22, Iss 4214, p 4214 (2021), International Journal of Molecular Sciences, Volume 22, Issue 8
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- Plants lack a circulating adaptive immune system to protect themselves against pathogens. Therefore, they have evolved an innate immune system based upon complicated and efficient defense mechanisms, either constitutive or inducible. Plant defense responses are triggered by elicitors such as microbe-associated molecular patterns (MAMPs). These components are recognized by pattern recognition receptors (PRRs) which include plant cell surface receptors. Upon recognition, PRRs trigger pattern-triggered immunity (PTI). Ethylene Inducing Xylanase (EIX) is a fungal MAMP protein from the plant-growth-promoting fungi (PGPF)–Trichoderma. It elicits plant defense responses in tobacco (Nicotiana tabacum) and tomato (Solanum lycopersicum), making it an excellent tool in the studies of plant immunity. Xylanases such as EIX are hydrolytic enzymes that act on xylan in hemicellulose. There are two types of xylanases: the endo-1, 4-β-xylanases that hydrolyze within the xylan structure, and the β-d-xylosidases that hydrolyze the ends of the xylan chain. Xylanases are mainly synthesized by fungi and bacteria. Filamentous fungi produce xylanases in high amounts and secrete them in liquid cultures, making them an ideal system for xylanase purification. Here, we describe a method for cost- and yield-effective xylanase production from Trichoderma using wheat bran as a growth substrate. Xylanase produced by this method possessed xylanase activity and immunogenic activity, effectively inducing a hypersensitive response, ethylene biosynthesis, and ROS burst.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Hypersensitive response
QH301-705.5
Nicotiana tabacum
enzyme purification
01 natural sciences
Catalysis
Article
Fungal Proteins
Inorganic Chemistry
03 medical and health sciences
Solanum lycopersicum
Tobacco
Plant defense against herbivory
Plant Immunity
Physical and Theoretical Chemistry
Biology (General)
Molecular Biology
innate immunity
QD1-999
Spectroscopy
Plant Proteins
Trichoderma
xylanase
Innate immune system
biology
Chemistry
Organic Chemistry
fungi
Pattern recognition receptor
food and beverages
General Medicine
Ethylenes
biology.organism_classification
Xylan
Computer Science Applications
Xylosidases
030104 developmental biology
Biochemistry
Xylanase
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 16616596 and 14220067
- Volume :
- 22
- Issue :
- 4214
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....94a6dd7fe6c577de612e093f1871b4ce