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In vitro cytotoxicity and virucidal efficacy of potassium hydrogen peroxymonosulfate compared to quaternary ammonium compound under various concentrations, exposure times and temperatures against African swine fever virus
- Source :
- Veterinary World, Veterinary World, Vol 14, Iss 11, Pp 2936-2940 (2021)
- Publication Year :
- 2021
- Publisher :
- Veterinary World, 2021.
-
Abstract
- Background and Aim: The selection and proper application of disinfectants are crucial to the prevention of many diseases, so disinfectants must be evaluated before being used for the prevention of African swine fever (ASF). Three disinfectant products belonging to the group of potassium hydrogen peroxymonosulfates, product A and product B, and a quaternary ammonium compound called product C, were examined in vitro for host cell cytotoxicity and the efficacy of ASF virus inactivation. The study parameters included various concentrations, exposure times, temperatures, and degrees of cytotoxicity. Materials and Methods: Three disinfectant products were evaluated for cytotoxicity using primary porcine alveolar macrophage (PAM) cells at dilutions from 1:200 to 1:51,200. Disinfectants in concentrations of 1:200, 1:400, and 1:800 were prepared, the pH and the virucidal activity were tested. An equal volume of each dilution was mixed with the ASF virus and incubated at room temperature (20°C) or on ice (4°C) for 1 min, 5 min, or 30 min. Hemadsorption (HAD) or rosette formation was observed using an inverted microscope for 5 days after inoculation, and the virus titer was calculated as HAD50/mL. Each treatment and virus control were tested in triplicate, and the titers were reported as means and standard deviations. The reduction factor was used to measure inactivation. Results: Products A, B, and C at 1:400, 1:800, and 1:25,600 of dilution, respectively, did not show significant cytotoxic effects on PAM cells. Products A and B could inactivate ASF virus at 1:200 dilution within 5 min after exposure at 4°C. However, at 20°C, the exposure time had to be extended to 30 min to inactivate the virus. Product C could inactivate the virus at 1:400 dilution within 5 min under both temperature conditions, whereas at 1:800 dilution, the exposure time had to be extended to 30 min to completely inactivate the virus at 20°C. Conclusion: All disinfectants could inactivate ASF virus in various concentrations, under appropriate exposure times and reaction temperatures, and there was no evidence of host cell cytotoxicity. For the control of ASF in pig farms, the appropriate concentration, ambient temperature, and contact time of these disinfectants should be taken into account.
- Subjects :
- General Veterinary
biology
Hydrogen
Veterinary medicine
Potassium
In vitro cytotoxicity
chemistry.chemical_element
biology.organism_classification
SF1-1100
African swine fever virus
Animal culture
chemistry.chemical_compound
chemistry
potassium hydrogen peroxymonosulfate
SF600-1100
porcine alveolar macrophage cell
quaternary ammonium compound
Ammonium
African swine fever
disinfectant
virucidal efficacy
Research Article
Nuclear chemistry
Subjects
Details
- ISSN :
- 22310916 and 09728988
- Database :
- OpenAIRE
- Journal :
- Veterinary World
- Accession number :
- edsair.doi.dedup.....94974c1a23bf21741503f534cdc9b765