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Effects of intracerebroventricular galanin or a galanin receptor 2/3 agonist on the lesion induced by transient occlusion of the middle cerebral artery in female rats
- Source :
- Neuropeptides. 45(1)
- Publication Year :
- 2010
-
Abstract
- Several studies have shown that injury to the central and peripheral nervous system can increase expression of galanin, a 29 amino acid neuropeptide. Moreover, there is evidence that galanin, especially through its galanin receptor 2 (GalR2) receptor, plays a neuroprotective role in different injury models. However, direct studies of a possible neuroprotective effect of galanin in experimental stroke models are lacking. Galanin, a GalR2/3 agonist or artificial CSF was continuously infused intracerebroventricularly (i.c.v.) in naive female rats after a 60min transient and focal occlusion of the middle cerebral artery. The animals were sacrificed, and the ischemic lesion was visualized using 2,3,5-triphenyltetrazolium hydrochloride (TTC) staining. The lesion was 98% larger after i.c.v. administration of the GalR2/3 agonist (2.4nmol/day) seven days after occlusion compared to artificial CSF (p=0.023). No statistically significant differences were found after seven days in the groups treated with galanin in three different concentrations (0.24, 2.4 and 24nmol/day; p=0.939, 0.715 and 0.977, respectively). There was no difference in the size of the ischemic lesions measured after three days in the galanin-treated group (2.4nmol/d) compared to artificial CSF (p=0.925). The present results show, surprisingly, that a GalR2/3 agonist doubled the size of the ischemic lesion. Whether this effect primarily reflects the properties of the current model, species, gender and/or the mode of galanin administration, e.g. causing desensitization, or whether galanin indeed lacks neuroprotective effect of its own, remains to be corroborated.
- Subjects :
- Agonist
medicine.medical_specialty
Middle Cerebral Artery
medicine.drug_class
Neuropeptide
Galanin receptor
Galanin
Lesion
Brain ischemia
Rats, Sprague-Dawley
Cellular and Molecular Neuroscience
Endocrinology
Internal medicine
medicine
Animals
Endocrine and Autonomic Systems
business.industry
digestive, oral, and skin physiology
Brain
Receptor, Galanin, Type 3
Infarction, Middle Cerebral Artery
General Medicine
medicine.disease
Rats
Receptor, Galanin, Type 2
medicine.anatomical_structure
Infusions, Intraventricular
Neurology
Peripheral nervous system
Female
medicine.symptom
business
Galanin receptor 2
Subjects
Details
- ISSN :
- 15322785
- Volume :
- 45
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Neuropeptides
- Accession number :
- edsair.doi.dedup.....9488eb7dc41c043e78049a29f7aaae7b