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Multiple connexin expression in regenerating arterial endothelial gap junctions
- Source :
- Arteriosclerosis, thrombosis, and vascular biology. 20(7)
- Publication Year :
- 2000
-
Abstract
- Abstract —Endothelial cells form gap junctions that, according to vessel type, may be composed of up to 3 types of connexin, connexin37, connexin40, and connexin43. Although changes in connexin expression have been linked to growth and injury in cultured endothelial cells, information on connexin expression in regenerating endothelium in situ is lacking. We investigated gap junction distribution and expression of all 3 endothelial connexins during healing in rat carotid artery after denudation injury. En face viewing of the vascular luminal surface by means of immunoconfocal microscopy was used to examine the spatial and temporal expression pattern of the endothelial connexins. Gap junction spots labeled by specific antisera against connexin37, connexin40, and connexin43 were quantified 7, 14, and 28 days after injury, and the relations among the connexins were examined by using colocalization analysis. Complementary electron microscopy was also conducted. After injury, the regenerating endothelium initially expressed small, sparse gap junctions, the numbers of which progressively increased to values equivalent to those of controls. Although connexin40 gap-junctional spot size and area returned to uninjured levels by 28 days after injury, connexin37 and connexin43 spot size and area exceeded those of the uninjured artery ( P 80% in the intact artery at postinjury day 28 and P
- Subjects :
- Male
Pathology
medicine.medical_specialty
Endothelium
Connexin
Biology
Antibodies
Connexins
Muscle, Smooth, Vascular
Rats, Sprague-Dawley
medicine
Animals
Wound Healing
Microscopy, Confocal
Gap junction
Colocalization
Gap Junctions
Rats
Endothelial stem cell
Microscopy, Electron
medicine.anatomical_structure
Connexin 43
Circulatory system
Endothelium, Vascular
Cardiology and Cardiovascular Medicine
Carotid Artery Injuries
Blood vessel
Artery
Subjects
Details
- ISSN :
- 10795642
- Volume :
- 20
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Arteriosclerosis, thrombosis, and vascular biology
- Accession number :
- edsair.doi.dedup.....9483d54c0e0a3ccc2d8372c3909dbadb