Supplementary Figures S1-S8 from Three-Dimensional Cell Culture-Based Screening Identifies the Anthelmintic Drug Nitazoxanide as a Candidate for Treatment of Colorectal Cancer

Supplementary Figures S1-S8. S1. Glucose concentration and pH in spheroid culture medium; S2. Dose-response and time-course experiment; S3. GFP-based and TOX8 assay comparison; S4. HT-29 spheroid-based clonogenic assay; S5. Final hit compounds' effects in spheroids formed with or without medium change S6. Nitazoxanide and tizoxanide effects comparison; S7. Nitazoxanide and irinotecan combination in clonogenic assay; S8. CD44 IHC staining.