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Characterisation of hydrocarbon degradation, biosurfactant production, and biofilm formation in Serratia sp. Tan611: a new strain isolated from industrially contaminated environment in Algeria
- Source :
- Antonie van Leeuwenhoek, Antonie van Leeuwenhoek, Springer Verlag, 2021, 114 (4), pp.411-424. ⟨10.1007/s10482-021-01527-5⟩
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- A novel bacterial strain was isolated from industrially contaminated waste water. In the presence of crude oil, this strain was shown to reduce the rate of total petroleum hydrocarbons (TPH) up to 97.10% in 24 h. This bacterium was subsequently identified by 16S rRNA gene sequence analysis and affiliated to the Serratia genus by the RDP classifier. Its genome was sequenced and annotated, and genes coding for catechol 1,2 dioxygenase and naphthalene 1,2-dioxygenase system involved in aromatic hydrocarbon catabolism, and LadA-type monooxygenases involved in alkane degradation, were identified. Gas Chromatography-Mass Spectrometry (GC-MS) analysis of crude oil after biological treatment showed that Serratia sp. Tan611 strain was able to degrade n-alkanes (from C-13 to C-25). This bacterium was also shown to produce a biosurfactant, the emulsification index (E24) reaching 43.47% and 65.22%, against vegetable and crude oil, respectively. Finally, the formation of a biofilm was increased in the presence of crude oil. These observations make Serratia sp. Tan611 a good candidate for hydrocarbon bioremediation.
- Subjects :
- Aromatic hydrocarbon catabolism
Serratia
[SDV.BID]Life Sciences [q-bio]/Biodiversity
Microbiology
Total petroleum hydrocarbons (TPH)
03 medical and health sciences
Bioremediation
Dioxygenase
RNA, Ribosomal, 16S
Food science
Molecular Biology
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
biology
Strain (chemistry)
030306 microbiology
Biofilm
Biosurfactant
General Medicine
biology.organism_classification
16S ribosomal RNA
Hydrocarbons
6. Clean water
Biodegradation, Environmental
Petroleum
Hydrocarbon
chemistry
13. Climate action
Algeria
Biofilms
n-alkanes
Bacteria
Subjects
Details
- ISSN :
- 15729699 and 00036072
- Volume :
- 114
- Database :
- OpenAIRE
- Journal :
- Antonie van Leeuwenhoek
- Accession number :
- edsair.doi.dedup.....94511d7f3e2629aa39d24dbb31c899cb