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Temporally regulated expression of Cre recombinase in neural stem cells
- Source :
- Genesis (New York, N.Y. : 2000). 41(4)
- Publication Year :
- 2005
-
Abstract
- The use of mouse gene targeting to study molecules important in neural development is oftentimes impaired by early embryonic lethality. In order to address later roles for such molecules, specifically in neural stem cells, we generated transgenic mice that express both the tetracycline-inducible molecule rtTA-M2 and GFP under the control of the neural precursor specific form of nestin. Developmental analysis of these mice demonstrates that GFP expression is exclusive to the neural tube. Adult expression of GFP is seen only in known areas of adult neurogenesis, namely, the subventricular zone and the dentate gyrus. When crossed with a second transgenic mouse (TetOp-Cre) that expresses the Cre recombinase under the control of the tetracycline responsive promotor, we demonstrate temporal induction of Cre in bigenic animals exposed to doxycycline. We further demonstrate the feasibility of this approach by using the ROSA-26 reporter mouse to mediate recombination in neural precursor cells.
- Subjects :
- Genetically modified mouse
Central Nervous System
Cre recombinase
Subventricular zone
Mice, Transgenic
Biology
Mice
Endocrinology
Genes, Reporter
Genetics
medicine
Animals
Promoter Regions, Genetic
Neurons
Integrases
Stem Cells
Neurogenesis
Neural tube
Gene Expression Regulation, Developmental
Cell Biology
Nestin
Tetracycline
Molecular biology
Neural stem cell
Anti-Bacterial Agents
medicine.anatomical_structure
Neural development
Subjects
Details
- ISSN :
- 1526954X
- Volume :
- 41
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Genesis (New York, N.Y. : 2000)
- Accession number :
- edsair.doi.dedup.....944460ffc6dda8b9e4fbb597c65ffee3