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Cytoglobin Is a Stress-responsive Hemoprotein Expressed in the Developing and Adult Brain
- Publication Year :
- 2006
- Publisher :
- SAGE Publications, 2006.
-
Abstract
- SUMMARY Cytoglobin (Cygb) is a novel tissue hemoprotein relatively similar to myoglobin (Mb). Because Cygb shares several structural features with Mb, we hypothesized that Cygb functions in the modulation of oxygen and nitric oxide metabolism or in scavenging free radicals within a cell. In the present study we examined the spatial and temporal expression pattern of Cygb during murine embryogenesis. Using in situ hybridization, RT-PCR, and Northern blot analyses, limited Cygb expression was observed during embryogenesis compared with Mb expression. Cygb expression was primarily restricted to the central nervous system and neural crest derivatives during the latter stages of development. In the adult mouse, Cygb is expressed in distinct regions of the brain as compared with neuroglobin (Ngb), another globin protein, and these regions are responsive to oxidative stress (i.e., hippocampus, thalamus, and hypothalamus). In contrast to Ngb, Cygb expression in the brain is induced in response to chronic hypoxia (10% oxygen). These results support the hypothesis that Cygb is an oxygen-responsive tissue hemoglobin expressed in distinct regions of the normoxic and hypoxic brain and may play a key role in the response of the brain to a hypoxic insult. (J Histochem Cytochem 54:1349–1361, 2006)
- Subjects :
- Histology
Central nervous system
Neuroglobin
Nerve Tissue Proteins
In situ hybridization
Biology
Sensitivity and Specificity
Article
Mice
medicine
Animals
Northern blot
Globin
Mice, Inbred ICR
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Profiling
Neurogenesis
Cytoglobin
Brain
Nuclear Proteins
Cell biology
Globins
Gene expression profiling
Mice, Inbred C57BL
medicine.anatomical_structure
Biochemistry
Spinal Cord
Anatomy
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....94140682f4c0197a43d157b7a05adaf4