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A pre-ribosome-associated HEAT-repeat protein is required for export of both ribosomal subunits

Authors :
Marlene Oeffinger
David Tollervey
Mensur Dlakić
Source :
Oeffinger, M, Dlakic, M & Tollervey, D 2004, ' A pre-ribosome-associated HEAT-repeat protein is required for export of both ribosomal subunits ', Genes & Development, vol. 18, no. 2, pp. 196-209 . https://doi.org/10.1101/gad.285604
Publication Year :
2004
Publisher :
Cold Spring Harbor Laboratory, 2004.

Abstract

Rrp12p (Ypl012w) is unusual among characterized ribosome synthesis factors in being associated with late precursors to both the 40S and 60S subunits. Rrp12p is predominately nuclear with nucleolar enrichment at steady state, but shuttled between the nucleus and cytoplasm in a heterokaryon assay. Strains depleted of Rrp12p are impaired in the nuclear export of both ribosomal subunits. Sequence analysis combined with fold recognition and modeling showed that Rrp12p is a member of a family of pre-ribosome-associated HEAT-repeat proteins. Like other HEAT-repeat transport factors, Rrp12p binds in vitro to nucleoporin FG-repeats of both the GLFG and FXFG families and to the GTPase Gsp1p (yeast RAN). Rrp12p also showed robust in vitro binding to a pre-rRNA transcript, in addition to poly(A) and poly(U). We propose that Rrp12p binds to the RNA components of the pre-ribosomes and promotes export of both subunits via its interactions with the nucleoporins and Gsp1p.

Details

ISSN :
15495477 and 08909369
Volume :
18
Database :
OpenAIRE
Journal :
Genes & Development
Accession number :
edsair.doi.dedup.....940fee6e0ca058dca205969afcf7f052
Full Text :
https://doi.org/10.1101/gad.285604