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A pre-ribosome-associated HEAT-repeat protein is required for export of both ribosomal subunits
- Source :
- Oeffinger, M, Dlakic, M & Tollervey, D 2004, ' A pre-ribosome-associated HEAT-repeat protein is required for export of both ribosomal subunits ', Genes & Development, vol. 18, no. 2, pp. 196-209 . https://doi.org/10.1101/gad.285604
- Publication Year :
- 2004
- Publisher :
- Cold Spring Harbor Laboratory, 2004.
-
Abstract
- Rrp12p (Ypl012w) is unusual among characterized ribosome synthesis factors in being associated with late precursors to both the 40S and 60S subunits. Rrp12p is predominately nuclear with nucleolar enrichment at steady state, but shuttled between the nucleus and cytoplasm in a heterokaryon assay. Strains depleted of Rrp12p are impaired in the nuclear export of both ribosomal subunits. Sequence analysis combined with fold recognition and modeling showed that Rrp12p is a member of a family of pre-ribosome-associated HEAT-repeat proteins. Like other HEAT-repeat transport factors, Rrp12p binds in vitro to nucleoporin FG-repeats of both the GLFG and FXFG families and to the GTPase Gsp1p (yeast RAN). Rrp12p also showed robust in vitro binding to a pre-rRNA transcript, in addition to poly(A) and poly(U). We propose that Rrp12p binds to the RNA components of the pre-ribosomes and promotes export of both subunits via its interactions with the nucleoporins and Gsp1p.
- Subjects :
- Saccharomyces cerevisiae Proteins
Nuclear Proteins
RNA
Saccharomyces cerevisiae
Sequence Analysis, DNA
Ribosomal RNA
Biology
Research Papers
Ribosome
Ribosome export
Protein Structure, Tertiary
Nuclear Pore Complex Proteins
Biochemistry
Ribosomal protein
Ran
RNA, Ribosomal, 18S
Genetics
Eukaryotic Small Ribosomal Subunit
Nucleoporin
Nuclear export signal
Ribosomes
Monomeric GTP-Binding Proteins
Developmental Biology
Subjects
Details
- ISSN :
- 15495477 and 08909369
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Genes & Development
- Accession number :
- edsair.doi.dedup.....940fee6e0ca058dca205969afcf7f052
- Full Text :
- https://doi.org/10.1101/gad.285604