Lymphocyte gating strategy. from Combination Therapy with Anti-PD-1, Anti-TIM-3, and Focal Radiation Results in Regression of Murine Gliomas

Cells were first gated on size and singularity by forward scatter and side scatter. Nonviable cells were excluded by live/dead gating. CD3+ live cells were gated on CD4 and CD8. CD3+FoxP3+CD4+ cells were designated as Tregs. TIM-3 vs. PD-1 expression was assessed for each lymphocyte population.