Multi-and transgenerational synergistic effects of glyphosate and chlorpyrifos at environmentally relevant concentrations in the estuarine rotifer Proales similis

We evaluated the multi-and transgenerational effects of single and combined environmentally relevant concentrations of glyphosate (GLY) and chlorpyrifos (CPF) in the estuarine rotifer Proales similis. The acute and chronic toxicities of GLY and CPF were determined as individual compounds and as a mixture. Rotifers were exposed to environmental concentrations of GLY (1, 10, 100, and 1000 μg/L) and CPF (0.1, 1, 5, and 10 μg/L). The main findings were as follows: (i) the LC