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Dl-3-n-butylphthalide regulates cholinergic dysfunction in chronic cerebral hypoperfusion rats
- Source :
- Journal of International Medical Research, Vol 48 (2020), The Journal of International Medical Research
- Publication Year :
- 2020
- Publisher :
- SAGE Publications, 2020.
-
Abstract
- Objectives To investigate whether dl-3-n-butylphthalide (NBP) affects cholinergic system function and ameliorates cognitive decline in a rat model of vascular dementia (VaD). Methods The VaD rat model was established by bilateral common carotid artery ligation (two-vessel occlusion, 2VO). Rats were divided into five groups: control, sham, 2VO, 2VO+NBP (80 mg/kg; intragastric), and 2VO+donepezil (1 mg/kg; intragastric). Treatments were administered once daily for 2 weeks from day 21 post-surgery. Spatial learning and memory were evaluated by Morris water maze performance. Hippocampal choline acetyltransferase (ChAT), acetylcholinesterase (AChE), vesicular acetylcholine transporter (VAChT), vascular endothelial growth factor (VEGF), and brain-derived neurotrophic factor (BDNF) expressions were detected using immunohistochemistry, immunofluorescence, and real-time polymerase chain reaction methods. Results The daily escape latency was significantly longer in 2VO rats than in the sham or control groups, while the time spent in the target quadrant was significantly shorter. The daily escape latency of the 2VO+NBP group was significantly shorter compared with the 2VO group. Following NBP treatment, ChAT, AChE, VAChT, and BDNF expressions were significantly upregulated in the hippocampus. Conclusions Central cholinergic dysfunction may be involved in VaD pathogenesis. NBP treatment significantly improved spatial learning and memory in VaD rats, and may enhance cholinergic system function via BDNF-mediated neuroprotection.
- Subjects :
- Male
Vascular Endothelial Growth Factor A
0301 basic medicine
Medicine (General)
Cholinergic Agents
Pharmacology
Hippocampus
Biochemistry
Brain Ischemia
Rats, Sprague-Dawley
chemistry.chemical_compound
0302 clinical medicine
Cognitive decline
Dl 3 n butylphthalide
vesicular acetylcholine transporter
acetylcholinesterase
General Medicine
Choline acetyltransferase
Acetylcholinesterase
Cholinergic Neurons
Acetylcholine
medicine.drug
China
Choline O-Acetyltransferase
Pre-Clinical Research Report
03 medical and health sciences
dl-3-n-butylphthalide
R5-920
Memory
Vesicular acetylcholine transporter
medicine
Animals
Cognitive Dysfunction
Maze Learning
Vascular dementia
Benzofurans
business.industry
Dementia, Vascular
Biochemistry (medical)
vascular dementia
Cell Biology
medicine.disease
choline acetyltransferase
acetylcholine
Rats
Disease Models, Animal
Oxidative Stress
030104 developmental biology
chemistry
Cholinergic
business
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 14732300 and 03000605
- Volume :
- 48
- Database :
- OpenAIRE
- Journal :
- Journal of International Medical Research
- Accession number :
- edsair.doi.dedup.....93f9d3f96860eea6f72a5904a7bd7d25