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Screening and identification of critical transcription factors involved in the protection of cardiomyocytes against hydrogen peroxide-induced damage by Yixin-shu
- Source :
- Scientific Reports, Vol 7, Iss 1, Pp 1-16 (2017), Scientific Reports
- Publication Year :
- 2017
- Publisher :
- Nature Publishing Group, 2017.
-
Abstract
- Oxidative stress initiates harmful cellular responses, such as DNA damage and protein denaturation, triggering a series of cardiovascular disorders. Systematic investigations of the transcription factors (TFs) involved in oxidative stress can help reveal the underlying molecular mechanisms and facilitate the discovery of effective therapeutic targets in related diseases. In this study, an integrated strategy which integrated RNA-seq-based transcriptomics techniques and a newly developed concatenated tandem array of consensus TF response elements (catTFREs)-based proteomics approach and then combined with a network pharmacology analysis, was developed and this integrated strategy was used to investigate critical TFs in the protection of Yixin-shu (YXS), a standardized medical product used for ischaemic heart disease, against hydrogen peroxide (H2O2)-induced damage in cardiomyocytes. Importantly, YXS initiated biological process such as anti-apoptosis and DNA repair to protect cardiomyocytes from H2O2-induced damage. By using the integrated strategy, DNA-(apurinic or apyrimidinic site) lyase (Apex1), pre B-cell leukemia transcription factor 3 (Pbx3), and five other TFs with their functions involved in anti-oxidation, anti-apoptosis and DNA repair were identified. This study offers a new understanding of the mechanism underlying YXS-mediated protection against H2O2-induced oxidative stress in cardiomyocytes and reveals novel targets for oxidative stress-related diseases.
- Subjects :
- 0301 basic medicine
Male
Transcription, Genetic
DNA repair
DNA damage
Cell Survival
Drug Evaluation, Preclinical
lcsh:Medicine
Apoptosis
Biology
medicine.disease_cause
Proteomics
Article
Cell Line
Rats, Sprague-Dawley
03 medical and health sciences
Transcription (biology)
Proto-Oncogene Proteins
medicine
DNA-(Apurinic or Apyrimidinic Site) Lyase
Animals
Humans
AP site
Myocytes, Cardiac
lcsh:Science
Transcription factor
Homeodomain Proteins
Multidisciplinary
lcsh:R
Hydrogen Peroxide
Molecular biology
DNA-(apurinic or apyrimidinic site) lyase
Cell biology
Rats
Oxidative Stress
030104 developmental biology
Cytoprotection
lcsh:Q
Transcriptome
Oxidative stress
Drugs, Chinese Herbal
Transcription Factors
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 7
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....93ee19aa4e881478f52bb3d61c2eb8e3