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Unique hepatitis B virus subgenotype in a primitive tribal community in eastern India
- Source :
- Journal of clinical microbiology. 48(11)
- Publication Year :
- 2010
-
Abstract
- Hepatitis B virus (HBV) strains isolated from members of the primitive Paharia ethnic community of Eastern India were studied to gain insight into the genetic diversity and evolution of the virus. The Paharia tribe has remained quite separate from the rest of the Indians and differs culturally, genetically, and linguistically from the mainstream East Indian population, whose HBV strains were previously characterized. Full-length HBV DNA was PCR amplified, cloned, and sequenced. Phylogenetic relationships between the tribal sequences and reference sequences from the mainstream population were assessed, and divergence times of subgenotypes of HBV genotype D were estimated. HBV was found in 2% of the Paharias participating in the study. A predominance of hepatitis B e antigen-negative infection (73%) was observed among the Paharias, and the genome sequences of the HBV strains exhibited relative homogeneity, with a very low prevalence of mutations. The novel feature of Paharia HBV was the exclusive presence of the D5 subgenotype, which was recently identified in Eastern India. Analysis of the four open reading frames (ORFs) of these tribal HBV D5 sequences and comparison with previously reported D1 to D7 sequences enabled the identification of 27 specific amino acid residues, including 6 unique ones, that could be considered D5 signatures. The estimated divergence times among subgenotypes D1 to D5 suggest that D5 was the first to diverge and hence is the most ancient of the D subgenotypes. The presence of a specific, ancient subgenotype of HBV within an ethnically primitive, endogamous population highlights the importance of studies of HBV genetics in well-separated human populations to understand viral transmission between communities and genome evolution.
- Subjects :
- Microbiology (medical)
Adult
Male
Genome evolution
Hepatitis B virus
Adolescent
Genotype
Sequence analysis
Epidemiology
Population
Molecular Sequence Data
India
Biology
medicine.disease_cause
Polymerase Chain Reaction
Young Adult
Population Groups
medicine
Cluster Analysis
Humans
Cloning, Molecular
education
Child
Phylogeny
Genetics
education.field_of_study
Molecular epidemiology
Phylogenetic tree
Genetic Variation
Sequence Analysis, DNA
Middle Aged
biology.organism_classification
Hepatitis B
Hepadnaviridae
DNA, Viral
Female
Subjects
Details
- ISSN :
- 1098660X
- Volume :
- 48
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Journal of clinical microbiology
- Accession number :
- edsair.doi.dedup.....93ebcca02e09f68edbf4450b18450112