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Uncoupling of Myelin Assembly and Schwann Cell Differentiation by Transgenic Overexpression of Peripheral Myelin Protein 22
- Source :
- The Journal of Neuroscience. 20:4120-4128
- Publication Year :
- 2000
- Publisher :
- Society for Neuroscience, 2000.
-
Abstract
- We have generated previously transgenic rats that overexpress peripheral myelin protein 22 (PMP22) in Schwann cells. In the nerves of these animals, Schwann cells have segregated with axons to the normal 1:1 ratio but remain arrested at the promyelinating stage, apparently unable to elaborate myelin sheaths. We have examined gene expression of these dysmyelinating Schwann cells using semiquantitative reverse transcription-PCR and immunofluorescence analysis. Unexpectedly, Schwann cell differentiation appears to proceed normally at the molecular level when monitored by the expression of mRNAs encoding major structural proteins of myelin. Furthermore, an aberrant coexpression of early and late Schwann cell markers was observed. PMP22 itself acquires complex glycosylation, suggesting that trafficking of the myelin protein through the endoplasmic reticulum is not significantly impaired. We suggest that PMP22, when overexpressed, accumulates in a late Golgi–cell membrane compartment and uncouples myelin assembly from the underlying program of Schwann cell differentiation.
- Subjects :
- Male
Glycosylation
Proteolipid protein 1
Fluorescent Antibody Technique
Schwann cell
Biology
Endoplasmic Reticulum
Myelin assembly
Animals, Genetically Modified
Myelin
Nerve Fibers
Peripheral myelin protein 22
Gene expression
medicine
Animals
ARTICLE
Myelin Sheath
Reverse Transcriptase Polymerase Chain Reaction
General Neuroscience
Endoplasmic reticulum
Homozygote
Cell Differentiation
Molecular biology
Rats
Cell biology
medicine.anatomical_structure
Bromodeoxyuridine
Gene Expression Regulation
nervous system
RNA
Schwann Cells
Neurology (clinical)
Schwann cell differentiation
Neuroscience
Myelin Proteins
Subjects
Details
- ISSN :
- 15292401 and 02706474
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- The Journal of Neuroscience
- Accession number :
- edsair.doi.dedup.....93e7be28b5817e0ca1527d8d6159d70e