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Crystal structure of a novel homodimeric l‐ribulose 3‐epimerase from Methylomonus sp
- Source :
- FEBS Open Bio, FEBS Open Bio, Vol 11, Iss 6, Pp 1621-1637 (2021)
- Publication Year :
- 2021
- Publisher :
- John Wiley and Sons Inc., 2021.
-
Abstract
- d‐Allulose has potential as a low‐calorie sweetener which can suppress fat accumulation. Several enzymes capable of d‐allulose production have been isolated, including d‐tagatose 3‐epimerases. Here, we report the isolation of a novel protein from Methylomonas sp. expected to be a putative enzyme based on sequence similarity to ketose 3‐epimerase. The synthesized gene encoding the deduced ketose 3‐epimerase was expressed as a recombinant enzyme in Escherichia coli, and it exhibited the highest enzymatic activity toward l‐ribulose, followed by d‐ribulose and d‐allulose. The X‐ray structure analysis of l‐ribulose 3‐epimerase from Methylomonas sp. (MetLRE) revealed a homodimeric enzyme, the first reported structure of dimeric l‐ribulose 3‐epimerase. The monomeric structure of MetLRE is similar to that of homotetrameric l‐ribulose 3‐epimerases, but the short C‐terminal α‐helix of MetLRE is unique and different from those of known l‐ribulose 3 epimerases. The length of the C‐terminal α‐helix was thought to be involved in tetramerization and increasing stability; however, the addition of residues to MetLRE at the C terminus did not lead to tetramer formation. MetLRE is the first dimeric l‐ribulose 3‐epimerase identified to exhibit high relative activity toward d‐allulose.<br />d‐Allulose has potential as a low‐calorie sweetener which can suppress fat accumulation. The enzymes capable of d‐allulose production have attracted attention. Here, we report the X‐ray structure of a novel l‐ribulose 3‐epimerase from Methylomonas sp. (MetLRE) which also has enzymatic activity towards d‐allulose, showing that MetLRE is the first dimeric l‐ribulose 3‐epimerase identified to exhibit high relative activity toward d‐allulose.
- Subjects :
- 0301 basic medicine
Models, Molecular
QH301-705.5
Stereochemistry
Pentoses
Racemases and Epimerases
Sequence (biology)
d‐allulose
Methylomonas
Crystallography, X-Ray
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
chemistry.chemical_compound
d‐allulose 3‐epimerase
0302 clinical medicine
β/α‐barrel
Tetramer
Biology (General)
Gene
Research Articles
rare sugar
chemistry.chemical_classification
l‐ribulose 3 epimerase
C-terminus
Ribulose
X‐ray structure
Ketose
Rare sugar
030104 developmental biology
Enzyme
chemistry
030220 oncology & carcinogenesis
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 22115463
- Volume :
- 11
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- FEBS Open Bio
- Accession number :
- edsair.doi.dedup.....93e19a8b16a23f923261af3f306c0a40