Additional file 1 of VHL Ser65 mutations enhance HIF2α signaling and promote epithelial-mesenchymal transition of renal cancer cells

Additional file 1: Figure S1. (A) Quantitative of HIF-2α expression levels in 786-O cells stably expressing VHL WT and mutants, with or without treatment of MG132. The western blot images were scanning densitometric values were obtained using ImageJ software. HIF-2α Protein levels were normalized to the loading control β-ACTIN. (B) Interaction of HIF-2α with VHL-WT and mutants after MG132 treatment. (C) Western blot for HIF-2α expression in 786-O cells treated with or without hypoxia mimetic CoCl2 and DMOG. CoCl2 was used at 100 uM final concentration for 6 h, DMOG was used at 1 mM final concentration for 8 h. (D) Immunostaining of FLAG-VHL in 786-O cells with and without MG132 treatment, Scale bars, 50 μm.