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Morphologic alterations in cultured human synovial fibroblasts induced by blood mononuclear cells
- Source :
- Experimental cell biology. 52(6)
- Publication Year :
- 1984
-
Abstract
- Effects of peripheral blood mononuclear cells on cultured synovial fibroblasts were studied. When mononuclear cells from normal or rheumatoid blood were incubated on synovial fibroblast cultures, a part of the cells adhered to the fibroblasts. They were mainly T lymphocytes but also some B lymphocytes and monocytes. After a 10-hour incubation, adhered mononuclear cells induced morphologic alterations to synovial fibroblasts: appearance of stellate cells and thinning and branching of fibroblasts. No changes were seen when the cells were incubated in the presence of indomethacin. Cytotoxicity of peripheral blood mononuclear cells from 8 rheumatoid patients was also tested against three rheumatoid and three normal synovial fibroblast strains. Only 2 out of 48 combinations were cytotoxic. The potentially cytotoxic mononuclear cells were bound equally well to rheumatoid and control synovial fibroblast cultures.
- Subjects :
- Pathology
medicine.medical_specialty
Fibroblast cultures
Cell Communication
Peripheral blood mononuclear cell
Monocytes
Pathology and Forensic Medicine
Arthritis, Rheumatoid
Organ Culture Techniques
medicine
Cell Adhesion
Humans
Fibroblast
Molecular Biology
Cells, Cultured
Staining and Labeling
business.industry
Synovial Membrane
Cell Biology
General Medicine
Fibroblasts
medicine.disease
medicine.anatomical_structure
Cell culture
Rheumatoid arthritis
Synovial membrane
business
Subjects
Details
- ISSN :
- 03043568
- Volume :
- 52
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Experimental cell biology
- Accession number :
- edsair.doi.dedup.....93bcd8ee9d8eb2029f0afe753ef22dea