Supplementary Figure 2 from Rho Kinase Inhibitor Fasudil Suppresses the Vasculogenic Mimicry of B16 Mouse Melanoma Cells Both In Vitro and In Vivo

(A) HE staining of tissue section. A vessel formed with elongated endothelial cells was showed by a red arrow, moreover, the vessel lack of endothelial cells nearby was pointed out by a yellow arrow. Original magnification, ×400. (B) Plenty of blood sinusoids were observed in the CD31/PAS dual-stained sections. Original magnification, ×100. (C) Endothelial cell lined-blood vessels containing CD31-positive immunoreactivity (dark brown) . (D) A VM structure were strictly defined as PAS-positive (purple magenta) CD31-negative. Detached tumor cells (black arrows) reached the blood stream, and it is suggested that VM networks facilitate tumor metastasis. Original magnification, ×400.