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Histochemical study of detailed laticifer structure and rubber biosynthesis-related protein localization in Hevea brasiliensis using spectral confocal laser scanning microscopy
- Source :
- Planta. 230:215-225
- Publication Year :
- 2009
- Publisher :
- Springer Science and Business Media LLC, 2009.
-
Abstract
- In Hevea brasiliensis, laticifers produce and accumulate rubber particles. Despite observation using histochemical methods, development stage structure and structures with ceasing functions have rarely been described. Spectral confocal laser scanning microscopy with Nile red staining simplifies laticifer structure observation in tangential sections while enhancing the resolution. Laticifer and ray images were extracted from unmixed images and used to monitor changes during growth. A laticifer network structure developed from increased anastomoses between adjoining laticifers outside of the conducting phloem, but because of increased radial division and growth of rays, the network structure ruptured and disintegrated. We also investigated immunohistochemical localization of two rubber particle-associated proteins in the laticifers: small rubber particle protein (SRPP) and rubber elongation factor (REF). Mature bark test results show that SRPP is localized only in the laticifer layers in the conducting phloem; REF is localized in all laticifer layers. Because SRPP plays a positive role in rubber biosynthesis, results show that the rubber biosynthesis capability of laticifers is concentrated where rays and the sieve tube actively transport metabolites.
- Subjects :
- Plant Science
Biology
Models, Biological
complex mixtures
law.invention
chemistry.chemical_compound
Natural rubber
Confocal microscopy
law
Oxazines
Botany
Genetics
Sieve tube element
Plant Proteins
Microscopy, Confocal
Histocytochemistry
Nile red
Allergens
Antigens, Plant
biology.organism_classification
Protein subcellular localization prediction
chemistry
visual_art
Laticifer
Plant Bark
visual_art.visual_art_medium
Biophysics
Hevea
Rubber
Hevea brasiliensis
Phloem
Subjects
Details
- ISSN :
- 14322048 and 00320935
- Volume :
- 230
- Database :
- OpenAIRE
- Journal :
- Planta
- Accession number :
- edsair.doi.dedup.....93ae14f24a5578150448b137cfd706b4