Back to Search
Start Over
Recombineering using RecET in Corynebacterium glutamicum ATCC14067 via a self-excisable cassette
- Source :
- Scientific Reports, Vol 7, Iss 1, Pp 1-8 (2017), Scientific Reports
- Publication Year :
- 2017
- Publisher :
- Nature Portfolio, 2017.
-
Abstract
- Gene manipulation is essential for metabolic engineering and synthetic biology, but the current general gene manipulation methods are not applicable to the non-model strain Corynebacterium glutamicum (C. glutamicum) ATCC14067, which is used for amino acid production. Here, we report an effective and sequential deletion method for C. glutamicum ATCC14067 using the exonuclease-recombinase pair RecE + RecT (RecET) for recombineering via a designed self-excisable linear double-strand DNA (dsDNA) cassette, which contains the Cre/loxP system, to accomplish markerless deletion. To the best of our knowledge, this is the first effective and simple strategy for recombination with markerless deletion in C. glutamicum ATCC14067. This strategy provides a simple markerless deletion strategy for C. glutamicum and builds a solid basis for producer construction.
- Subjects :
- 0301 basic medicine
Genetics
Genetics, Microbial
Multidisciplinary
Science
030106 microbiology
Biology
Recombineering
Article
Corynebacterium glutamicum
Metabolic engineering
Recombinases
03 medical and health sciences
chemistry.chemical_compound
Synthetic biology
Gene Knockout Techniques
Exodeoxyribonucleases
chemistry
bacteria
Medicine
Gene
DNA
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 7
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....93ac270403812d226d2ffb9015e317ae