Combined effects of arbuscular mycorrhizae fungus and composted pig manure on the growth of ryegrass and uptake of Cd and Zn in the soil from an e-waste recycling site

Little attention has been paid to the combined effects of arbuscular mycorrhizae (AM) fungus and composted manure on heavy metal bioavailability and its uptake by plants grown in heavy metal-contaminated soils from electronic-waste (e-waste) recycling sites. A greenhouse pot experiment was conducted to investigate the effects of AM fungus, composted pig manure (CM) and AM fungus + CM (ACM) on the growth of ryegrass and uptake of Cd and Zn in the soil collected from an e-waste recycling site. The calcium chloride (CaCl2) and Tessier sequential extraction procedure were adopted to evaluate the bioavailability and chemical speciation of Cd and Zn in the soil. Results showed that the application of CM and ACM significantly increased the pH but decreased the CaCl2-extractable Cd and Zn concentrations in the rhizosphere and bulk soils. ACM treatment significantly shifted Cd from exchangeable fraction to other more stable fractions, and transformed the exchangeable Zn fraction to the carbonate-bound and reducible iron and manganese-bound fractions. Furthermore, the application of ACM can enhance the growth of plant shoots, and decrease the uptake of Cd and Zn in the ryegrass plants. This work suggests that AM fungus in combination with CM amendment may be a potential method for not only remediation of soil Cd and Zn pollution, but also reduction of Cd and Zn uptake by ryegrass grown in the soil from e-waste recycling sites.