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Analysis of in vivo chlorophyll fluorescence spectra to monitor physiological state of tomato plants growing under zinc stress
- Source :
- Journal of photochemistry and photobiology. B, Biology. 101(3)
- Publication Year :
- 2010
-
Abstract
- The effects of zinc (Zn) on plant chlorophyll fluorescence were investigated in 10-day-old tomato ( Solanum lycopersicum ) seedlings subjected for 7 days to a series of zinc (10, 50, 100 and 150 μM) applied via the nutrient solution. The chlorophyll fluorescence spectra of leaves were recorded in the spectral region 650–800 nm using the spectroscopic technique of ultraviolet light emitting diode induced fluorescence spectroscopy (UV-LED IFS). These spectra have been used to analyze the effect of several doses of zinc on the photosynthetic activities of tomato plants. The fluorescence intensity ratios (FIR) at the two maxima ( F 690 / F 735 ) of control as well as treated tomato plants were calculated by evaluating curve-fitted parameters using a Gaussian spectral function. The variable chlorophyll fluorescence decrease ratio ( R Fd ) values were determined from the fluorescence induction kinetics curves recorded at 690 nm and 735 nm. In addition, Zn accumulation in plants, plant growth, photosynthetic pigments content and malondialdehyde level (MDA, an index of lipid peroxidation) were also estimated. The results indicated that the plants treated with 10 μM of zinc exhibited better growth, however, higher concentrations of zinc were harmful for plants. Excess Zn induced a decrease in the R Fd values, which was associated with a strong decline of the total chlorophylls content and an increase of MDA level. The total chlorophylls content decline could also be followed via an increase of the chlorophyll fluorescence ratio F 690 / F 735 .
- Subjects :
- Chlorophyll
Biophysics
Analytical chemistry
chemistry.chemical_element
Zinc
Photosynthesis
Fluorescence spectroscopy
chemistry.chemical_compound
Pigment
Solanum lycopersicum
Malondialdehyde
Ultraviolet light
Radiology, Nuclear Medicine and imaging
Chlorophyll fluorescence
Radiation
Radiological and Ultrasound Technology
biology
Chemistry
fungi
food and beverages
Pigments, Biological
biology.organism_classification
Plant Leaves
Kinetics
Spectrometry, Fluorescence
visual_art
visual_art.visual_art_medium
Solanum
Nuclear chemistry
Subjects
Details
- ISSN :
- 18732682
- Volume :
- 101
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Journal of photochemistry and photobiology. B, Biology
- Accession number :
- edsair.doi.dedup.....93a1d4f486162171e3cd25db2963023f