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Validation of an Easy Handling Sample Preparation and Triplex Real Time PCR for Rapid Detection of T. equigenitalis and Other Organisms Associated with Endometritis in Mares
Validation of an Easy Handling Sample Preparation and Triplex Real Time PCR for Rapid Detection of T. equigenitalis and Other Organisms Associated with Endometritis in Mares
- Source :
- Journal of Equine Veterinary Science, Journal of Equine Veterinary Science, WB Saunders, 2020, 94, pp.103241. ⟨10.1016/j.jevs.2020.103241⟩
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- International audience; Isolation and identification of Taylorella equigenitalis, the causative agent of contagious equine metritis, by bacteriology is laborious and does not permit differentiation from the other member of the genus, Taylorella asinigenitalis. Moreover, other organisms such as Klebsiella pneumoniae and Pseudomonas aeruginosa can also cause endometritis in mares and warrant diagnostic detection. Our objectives were to develop a rapid preparation method for field swab samples and to validate this protocol using new multiplex real-time polymerase chain reaction (rtPCR) detection tools for identification of these four pathogens. The complete analytical process from sample preparation to PCR analysis was then evaluated against bacteriology, the World Organisation for Health's (OIE) gold standard method for T. equigenitalis and commonly used for the other three pathogens. The diagnostic sensitivity and specificity of this method, which used direct lysis and a multiplex rtPCR, were 100% and >92%, respectively. This study provided a simple-to-use method for prebreeding screening of mares and stallions.
- Subjects :
- Male
040301 veterinary sciences
Real-Time Polymerase Chain Reaction
law.invention
Microbiology
0403 veterinary science
Direct lysis
Contagious equine metritis
law
Taylorella asinigenitalis
Bacteriology
medicine
Animals
Taylorella
Multiplex
Horses
Diagnostics
Polymerase chain reaction
[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health
biology
Equine
0402 animal and dairy science
04 agricultural and veterinary sciences
Gold standard (test)
biology.organism_classification
medicine.disease
040201 dairy & animal science
3. Good health
Taylorella equigenitalis
Female
Horse Diseases
Endometritis
Gram-Negative Bacterial Infections
rtPCR
Subjects
Details
- ISSN :
- 07370806
- Volume :
- 94
- Database :
- OpenAIRE
- Journal :
- Journal of Equine Veterinary Science
- Accession number :
- edsair.doi.dedup.....9387a32544e272f38597a38f1e7ad26c
- Full Text :
- https://doi.org/10.1016/j.jevs.2020.103241