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Determination of the complexity and diversity of the TCR β‑chain CDR3 repertoire in bladder cancer using high‑throughput sequencing
- Source :
- Oncology Letters
- Publication Year :
- 2019
- Publisher :
- Spandidos Publications, 2019.
-
Abstract
- The present study aimed to investigate the complexity and diversity of the T lymphocyte immune repertoire in patients with bladder cancer. To do so, the immune state of patients was assessed. The study also aimed to elucidate the aetiology and pathogenesis of bladder cancer to provide a novel theoretical basis for disease prevention, diagnosis, treatment and prognosis monitoring. Cancerous and paracancerous (control) tissue samples were collected from five patients diagnosed with muscle-invasive bladder cancer. Multiplex polymerase chain reaction and Illumina high-throughput sequencing were used to determine the characteristics and clonal diversity of the T-cell receptor (TCR) β-chain complementarity-determining region 3 (CDR3) gene in the cancerous and paracancerous tissues of patients with bladder cancer. The degree of clonal expansion in malignant samples was significantly higher than in adjacent samples. Furthermore, ΤCRβ variable (TRBV), ΤCRβ diversity (TRBD) and ΤCRβ joining (TRBJ) repertoires were significantly different in cancerous samples compared with adjacent samples. In addition, 13 identified V-J pairs were highly expressed in cancerous samples whereas they had low expression in control samples. In conclusion, the degree of T-cell clonal expansion in bladder cancerous tissue was higher than in paracancerous tissue, whereas the immune diversity of the tissues of patients with bladder cancer was significantly lower. The DNA sequence and amino acid sequences, and V-J combination level may be used to comprehensively understand the diversity and characteristics of TCR CDR3 in bladder cancer and paracancerous tissues, and to evaluate the immune status of bladder cancer to develop therapeutic targets and biomarkers for prognosis monitoring.
- Subjects :
- 0301 basic medicine
Cancer Research
Bladder cancer
Oncogene
T-cell receptor
high-throughput sequencing
Cancer
Articles
Biology
medicine.disease
Molecular medicine
immune repertoire
DNA sequencing
03 medical and health sciences
030104 developmental biology
0302 clinical medicine
Immune system
Oncology
030220 oncology & carcinogenesis
Multiplex polymerase chain reaction
Cancer research
medicine
bladder cancer
tumour-infiltrating lymphocytes
Subjects
Details
- ISSN :
- 17921082 and 17921074
- Database :
- OpenAIRE
- Journal :
- Oncology Letters
- Accession number :
- edsair.doi.dedup.....936b92b86cb9be648163ab123bf75fa3