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Allele and genotype frequencies of the SOD1 gene polymorphism associated with canine degenerative myelopathy in Belgian Malinois dogs in Greece
- Source :
- Veterinary World, Vol 14, Iss 6, Pp 1472-1479 (2021)
- Publication Year :
- 2021
- Publisher :
- Veterinary World, 2021.
-
Abstract
- Background and Aim: Canine degenerative myelopathy (CDM) is an adult-onset fatal disorder associated with a point mutation of the superoxide dismutase 1 (SOD1) gene (SOD1:c.118G>A). This study aimed to determine the allele and genotype frequencies of this mutation in a group of Belgian Malinois dogs in Greece. Materials and Methods: Samples (n=72) of whole blood were collected from 72 purebred dogs of the Hellenic Armed Forces; these samples were processed for DNA isolation, polymerase chain reaction, and digestion with the restriction endonuclease AcuI. Sample testing was conducted in compliance with ISO17025 accreditation requirements. Results: The observed relative genotype frequencies were 71% for the homozygous (GG), 25% for the heterozygous (AG), and 4% for the homozygous mutant (AA) alleles. These frequencies were close to those expected, indicating no significant departure from Hardy–Weinberg equilibrium (HWE, p=0.395). The frequency of heterozygous animals indicates that a high risk of developing CDM in forthcoming generations exists in the tested population because mating among carriers would result in 25% AA progeny. The medical record of the group of study animals indicated selection against leishmaniosis, as applied throughout generations by owners and breeders. The potential association of this selection with the HWE status of the study population was discussed. Conclusion: The SOD1:c.118G>A mutation was common in the tested group of dogs; thus, they are suitable for a follow-up investigation on the development and progression of CDM. A case-control study on animals with evidence of sensitivity to infectious myelopathy could provide new insights into disease pathogenesis.
- Subjects :
- degenerative myelopathy
dogs
040301 veterinary sciences
Veterinary medicine
Population
genetic analysis
Biology
Canine degenerative myelopathy
SF1-1100
Belgian Malinois
0403 veterinary science
03 medical and health sciences
Myelopathy
SF600-1100
medicine
genetic polymorphism
restriction fragment length polymorphism-polymerase chain reaction
Allele
education
030304 developmental biology
Genetics
0303 health sciences
education.field_of_study
General Veterinary
sod1:c.118a
04 agricultural and veterinary sciences
medicine.disease
Genotype frequency
Animal culture
Population study
Gene polymorphism
Subjects
Details
- Language :
- English
- ISSN :
- 22310916 and 09728988
- Volume :
- 14
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Veterinary World
- Accession number :
- edsair.doi.dedup.....93585858eb2ec304d0b0828c6a39bc34