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Effect of Environmental Factors on the trans/cis Ratio of Unsaturated Fatty Acids in Pseudomonas putida S12
- Source :
- Applied and Environmental Microbiology 62 (1996), Applied and Environmental Microbiology, 62, 2773-2777
- Publication Year :
- 1996
- Publisher :
- American Society for Microbiology, 1996.
-
Abstract
- The membrane reactions of Pseudomonas putida S12 to environmental stress were investigated. Cells reacted to the addition of six different heavy metals with an increase in the ratio of trans to cis unsaturated fatty acids. A correlation among the increase in the trans/cis ratio, the toxic effects of the heavy metals, and nonspecific permeabilization of the cytoplasmic membrane, as indicated by an efflux of potassium ions, was measured. Cells previously adapted to toxic concentrations of toluene exhibited increased tolerance to all applied concentrations of zinc compared with nonadapted cells. Cells exposed to different temperatures grew optimally at 30(deg)C. The degree of saturation of the membrane fatty acids of these cells decreased with decreasing temperature. An increase in the trans/cis ratio of unsaturated fatty acids took place only at higher temperatures. Osmotic stress, expressed as reduced water activity, was obtained by using different types of solutes. Only in the presence of toxic concentrations of sodium chloride or sucrose did the trans/cis ratio increase. At no applied water activity a significant effect of glycerol on the trans/cis ratio was measured. When cells were exposed to different pHs, a distinct optimum cis/trans isomerase activity was measured at pHs between 4.0 and 5.0, whereas at higher or lower pHs no reaction occurred. This optimum coincided with a loss of viability between pH 4 and 5.
- Subjects :
- Isomerase activity
Ecology
biology
Osmotic shock
Sodium
chemistry.chemical_element
biology.organism_classification
Applied Microbiology and Biotechnology
Cis trans isomerization
Pseudomonas putida
Industrial Microbiology
chemistry.chemical_compound
chemistry
Biochemistry
Industriële microbiologie
Glycerol
Life Science
Unsaturated fatty acid
Cis–trans isomerism
VLAG
Research Article
Food Science
Biotechnology
Nuclear chemistry
Subjects
Details
- ISSN :
- 10985336 and 00992240
- Volume :
- 62
- Database :
- OpenAIRE
- Journal :
- Applied and Environmental Microbiology
- Accession number :
- edsair.doi.dedup.....9357a8239a18f8b8cb9a8b5a83f3d9f8
- Full Text :
- https://doi.org/10.1128/aem.62.8.2773-2777.1996