Back to Search
Start Over
Aryl hydrocarbon receptor (AhR)-dependent regulation of pulmonary miRNA by chronic cigarette smoke exposure
- Source :
- Scientific Reports
- Publication Year :
- 2017
- Publisher :
- Nature Publishing Group, 2017.
-
Abstract
- The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor historically known for its toxic responses to man-made pollutants such as dioxin. More recently, the AhR has emerged as a suppressor of inflammation, oxidative stress and apoptosis from cigarette smoke by mechanisms that may involve the regulation of microRNA. However, little is known about the AhR regulation of miRNA expression in the lung in response to inhaled toxicants. Therefore, we exposed Ahr−/− and Ahr+/− mice to cigarette smoke for 4 weeks and evaluated lung miRNA expression by PCR array. There was a dramatic regulation of lung miRNA by the AhR in the absence of exogenous ligand. In response to cigarette smoke, there were more up-regulated miRNA in Ahr−/− mice compared to Ahr+/− mice, including the cancer-associated miRNA miR-96. There was no significant change in the expression of the AhR regulated proteins HuR and cyclooxygenase-2 (COX-2). There were significant increases in the anti-oxidant gene sulfiredoxin 1 (Srxn1) and FOXO3a- predicted targets of miR-96. Collectively, these data support a prominent role for the AhR in regulating lung miRNA expression. Further studies to elucidate a role for these miRNA may further uncover novel biological function for the AhR in respiratory health and disease.
- Subjects :
- 0301 basic medicine
Inflammation
medicine.disease_cause
Article
ELAV-Like Protein 1
03 medical and health sciences
0302 clinical medicine
microRNA
medicine
Animals
Oxidoreductases Acting on Sulfur Group Donors
Receptor
Transcription factor
Lung
Regulation of gene expression
Mice, Knockout
Multidisciplinary
biology
Chemotaxis
Forkhead Box Protein O3
Smoking
respiratory system
Aryl hydrocarbon receptor
3. Good health
respiratory tract diseases
Mice, Inbred C57BL
Kinetics
MicroRNAs
030104 developmental biology
Gene Expression Regulation
Receptors, Aryl Hydrocarbon
13. Climate action
Apoptosis
Cyclooxygenase 2
030220 oncology & carcinogenesis
Cancer research
biology.protein
Cytokines
medicine.symptom
Oxidative stress
Gene Deletion
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....935591cfe5985af1e2256e0f6c21b4d8
- Full Text :
- https://doi.org/10.1038/srep40539