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Detection of Anti-Pentraxin-3 Autoantibodies in ANCA-Associated Vasculitis
- Source :
- PLoS ONE, PLoS ONE, Public Library of Science, 2016, 11 (1), pp.e0147091. ⟨10.1371/journal.pone.0147091⟩, PLoS ONE, 2016, 11 (1), pp.e0147091. ⟨10.1371/journal.pone.0147091⟩, PLoS ONE, Vol 11, Iss 1, p e0147091 (2016)
- Publication Year :
- 2016
- Publisher :
- Public Library of Science, 2016.
-
Abstract
- Objectives Pentraxin 3 (PTX3), in common with myeloperoxidase and proteinase 3, is stored in human neutrophil granules and is expressed on apoptotic neutrophil surface. We therefore investigated the presence of anti-PTX3 autoantibodies (aAbs) in the sera of antineutrophil cytoplasmic antibodies (ANCA)-associated vasculitis (AAV) patients. Methods Presence of anti-PTX3 autoantibodies was analysed by a specific enzyme-linked immunosorbent assay in sera from 150 patients with microscopic polyangiitis (MPA), granulomatosis with polyangiitis (GPA), and eosinophilic granulomatosis with polyangiitis (EGPA), and in sera of 227 healthy subjects (HS), 40 systemic sclerosis (SSc) patients, and 25 giant cell arteritis patients (GCA). Using indirect immunofluorescence on fixed human neutrophils, we also analyzed the staining pattern associated with the presence of anti-PTX3 aAbs. Results Anti-PTX3 aAbs were detected in 56 of 150 (37.3%) of the AAV patients (versus 12 of 227 (5.3%) of HS, p
- Subjects :
- 0301 basic medicine
Male
Pathology
Neutrophils
lcsh:Medicine
Apoptosis
Pathology and Laboratory Medicine
Biochemistry
White Blood Cells
0302 clinical medicine
Proteinase 3
Animal Cells
Medicine and Health Sciences
Enzyme-Linked Immunoassays
lcsh:Science
skin and connective tissue diseases
Fluorescent Antibody Technique, Indirect
Staining
Multidisciplinary
biology
Cell Death
Middle Aged
Prognosis
3. Good health
Serum Amyloid P-Component
C-Reactive Protein
Cell Processes
Myeloperoxidase
Female
Cellular Types
Microscopic polyangiitis
Granulomatosis with polyangiitis
Vasculitis
Research Article
Adult
medicine.medical_specialty
Immune Cells
Inflammatory Diseases
Immunology
[SDV.CAN]Life Sciences [q-bio]/Cancer
Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis
Enzyme-Linked Immunosorbent Assay
Research and Analysis Methods
Autoimmune Diseases
03 medical and health sciences
[SDV.CAN] Life Sciences [q-bio]/Cancer
Antigen
Rheumatology
medicine
Humans
cardiovascular diseases
Wegener Granulomatosis
Immunoassays
Cytoplasmic Staining
Anti-neutrophil cytoplasmic antibody
Autoantibodies
030203 arthritis & rheumatology
Blood Cells
business.industry
lcsh:R
Autoantibody
Biology and Life Sciences
Cell Biology
medicine.disease
030104 developmental biology
Specimen Preparation and Treatment
Case-Control Studies
biology.protein
Immunologic Techniques
lcsh:Q
Clinical Immunology
Clinical Medicine
business
Biomarkers
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 11
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....934af7c799166e3b1ce2f04b77a7a8c5