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Is retained bone debris in cannulated orthopedic instruments sterile after autoclaving?
- Source :
- American Journal of Infection Control. 46:1009-1013
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Aims Cannulated surgical instruments may retain biologic debris after routine cleaning and sterilization. Residual debris after cleaning is assumed to be sterile; however, there is no experimental basis for this assumption. The purpose of this study was to determine the sterility of retained biodebris found within cannulated surgical instruments after autoclave sterilization. Materials and Methods Fifteen cannulated drill bits were used to drill pig scapulae to create a plug of bone that was exposed to a mixture of Bacillus cereus, Pseudomonas aeruginosa, and methicillin-resistant Staphylococcus aureus for 60, 120, or 180 minutes prior to sterilization. The drill bits were autoclave sterilized using standard settings. The “sterilized” bone cores were then incubated in solution and streak-plated on blood agar. Results All 3 positive controls were positive for the experimental bacteria. Two negative controls were positive for contaminant bacteria. A B. cereus strain was recovered from 1 of the experimental group drill bits in the 180-minute group. Pulsed-field gel electrophoresis confirmed that the recovered B. cereus strain was identical to the experimental inoculate. Conclusion Retained biodebris in cannulated drills may not be sterile after standard autoclave sterilization. In addition, delay of surgical instrument reprocessing may increase the risk of resistant contamination.
- Subjects :
- Methicillin-Resistant Staphylococcus aureus
Genotype
Swine
Epidemiology
Bacillus cereus
Dentistry
Bone and Bones
Autoclave
Agar plate
03 medical and health sciences
0302 clinical medicine
Animals
Medicine
030212 general & internal medicine
Decontamination
030222 orthopedics
biology
Drill
business.industry
Health Policy
Public Health, Environmental and Occupational Health
Sterilization
Models, Theoretical
Sterilization (microbiology)
Surgical Instruments
biology.organism_classification
Electrophoresis, Gel, Pulsed-Field
Molecular Typing
Orthopedics
Infectious Diseases
Pseudomonas aeruginosa
business
Subjects
Details
- ISSN :
- 01966553
- Volume :
- 46
- Database :
- OpenAIRE
- Journal :
- American Journal of Infection Control
- Accession number :
- edsair.doi.dedup.....93405ac45f690a92170d8f5a66e74628