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Binding of Clitoria ternatea L. flower extract with α-amylase simultaneously monitored at two wavelengths using a photon streaming time-resolved fluorescence approach
- Source :
- Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 211
- Publication Year :
- 2018
-
Abstract
- The binding of an extract from the flowers of Clitoria ternatea L. to the digestive enzyme α-amylase was investigated. This extract is a mixture of flavonoids, including anthocyanins, and has been previously shown to inhibit the activity this enzyme. This has implications for modulating starch digestion. In order to investigate the kinetics, we made use of time-resolved fluorescence to simultaneously monitor two different emission bands emanating from the extract. This measurement was enabled by the use of a “photon streaming” approach and changes in fluorescence lifetime and intensity were used to follow the interaction. A longer wavelength band (655 nm) was ascribed to anthocyanins in the mixture and these were observed to bind at a rate an order of magnitude slower than other flavonoids present in the extract, monitored at a shorter wavelength (485 nm). Changes in the fluorescence emission of the extract upon binding were further assessed by the use of decay associated spectra.
- Subjects :
- Photon
Kinetics
Flowers
Analytical Chemistry
Anthocyanins
Amylase
Instrumentation
Spectroscopy
chemistry.chemical_classification
Flavonoids
Photons
Chromatography
biology
Plant Extracts
Clitoria ternatea
food and beverages
Starch
biology.organism_classification
Fluorescence
Atomic and Molecular Physics, and Optics
Wavelength
Enzyme
Spectrometry, Fluorescence
chemistry
biology.protein
Time-resolved spectroscopy
alpha-Amylases
Clitoria
Subjects
Details
- ISSN :
- 18733557
- Volume :
- 211
- Database :
- OpenAIRE
- Journal :
- Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
- Accession number :
- edsair.doi.dedup.....9317a22ecc98ec6fd89886f05f8ba255