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Reversion to virulence of a subtype B avian metapneumovirus vaccine: Is it time for regulators to require availability of vaccine progenitors?
- Publication Year :
- 2014
-
Abstract
- Empirically derived live avian metapneumovirus (AMPV) vaccines developed during the late 80s and early 90s have generally performed well in controlling turkey rhinotracheitis. Nonetheless, unstable attenuation was previously demonstrated in an AMPV subtype A vaccine. Until now this had not been investigated in subtype B vaccines due to lack of any similar availability of a vaccine progenitor or its sequence. The publication of the full genome sequence for the VCO3 vaccine progenitor facilitated a conclusive investigation of two AMPVs isolated from poults on a farm which had been vaccinated with VCO3 derived vaccine. Full genome sequencing of the isolates and their comparison to sequences of the vaccine and its progenitor, confirmed their vaccine origin. After determining the absence of extraneous infectious agents, one of these virus isolates was inoculated into 1-day-old turkeys in disease secure isolators and shown to cause disease with a severity similar to that caused by virulent field virus. This suggests that instability in live AMPV vaccines may be generalized and highlights the need for availability of vaccine progenitor sequences for the field assessment of all live viral vaccines.
- Subjects :
- Environmental vaccine identification
Turkeys
Turkey
Immunology and Microbiology (all)
Reversion
Virulence
Infectious Disease
Disease
Genome, Viral
Biology
Reversion to virulence
Vaccines, Attenuated
Virus
Avian metapneumoviru
Microbiology
Farm disease
Animals
Avian metapneumovirus
Metapneumovirus
Progenitor cell
AMPV subtype B
Poultry Diseases
Whole genome sequencing
Paramyxoviridae Infections
General Veterinary
General Immunology and Microbiology
Animal
Viral Vaccine
Medicine (all)
Public Health, Environmental and Occupational Health
Viral Vaccines
Sequence Analysis, DNA
Virology
Paramyxoviridae Infection
Infectious Diseases
Poultry Disease
Vaccine progenitor availability
Metapneumoviru
Veterinary (all)
Molecular Medicine
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....93018f4a20543709dd0724802ebb6cfe