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Dynamic Assessment of the Endothelialization of Tissue-Engineered Blood Vessels Using an Optical Coherence Tomography Catheter-Based Fluorescence Imaging System
- Source :
- Tissue engineering. Part C, Methods. 21(7)
- Publication Year :
- 2014
-
Abstract
- Lumen endothelialization of bioengineered vascular scaffolds is essential to maintain small-diameter graft patency and prevent thrombosis postimplantation. Unfortunately, nondestructive imaging methods to visualize this dynamic process are lacking, thus slowing development and clinical translation of these potential tissue-engineering approaches. To meet this need, a fluorescence imaging system utilizing a commercial optical coherence tomography (OCT) catheter was designed to visualize graft endothelialization.C7 DragonFly™ intravascular OCT catheter was used as a channel for delivery and collection of excitation and emission spectra. Poly-dl-lactide (PDLLA) electrospun scaffolds were seeded with endothelial cells (ECs). Seeded cells were exposed to Calcein AM before imaging, causing the living cells to emit green fluorescence in response to blue laser. By positioning the catheter tip precisely over a specimen using high-fidelity electromechanical components, small regions of the specimen were excited selectively. The resulting fluorescence intensities were mapped on a two-dimensional digital grid to generate spatial distribution of fluorophores at single-cell-level resolution. Fluorescence imaging of endothelialization on glass and PDLLA scaffolds was performed using the OCT catheter-based imaging system as well as with a commercial fluorescence microscope. Cell coverage area was calculated for both image sets for quantitative comparison of imaging techniques. Tubular PDLLA scaffolds were maintained in a bioreactor on seeding with ECs, and endothelialization was monitored over 5 days using the OCT catheter-based imaging system.No significant difference was observed in images obtained using our imaging system to those acquired with the fluorescence microscope. Cell area coverage calculated using the images yielded similar values. Nondestructive imaging of endothelialization on tubular scaffolds showed cell proliferation with cell coverage area increasing from 15 ± 4% to 89 ± 6% over 5 days.In this study, we showed the capability of an OCT catheter-based imaging system to obtain single-cell resolution and to quantify endothelialization in tubular electrospun scaffolds. We also compared the resulting images with traditional microscopy, showing high fidelity in image capability. This imaging system, used in conjunction with OCT, could potentially be a powerful tool for in vitro optimization of scaffold cellularization, ensuring long-term graft patency postimplantation.
- Subjects :
- Fluorescence-lifetime imaging microscopy
Materials science
Catheters
genetic structures
Biomedical Engineering
Medicine (miscellaneous)
Bioengineering
Fluorescence
Article
Tissue engineering
Optical coherence tomography
medicine
Humans
Cell Line, Transformed
Tissue engineered
medicine.diagnostic_test
Graft patency
Tissue Engineering
Tissue Scaffolds
eye diseases
Catheter
Blood Vessels
Tomography
Tomography, Optical Coherence
Lumen (unit)
Biomedical engineering
Subjects
Details
- ISSN :
- 19373392
- Volume :
- 21
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Tissue engineering. Part C, Methods
- Accession number :
- edsair.doi.dedup.....92f6bd38dc1218f2a67bed7fed3f1175