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Evaluation of the modified carbapenem inactivation method for the detection of carbapenemase-producing Enterobacteriaceae

Authors :
Akihiro Nakamura
Yasunao Wada
Tomokazu Kuchibiro
Masaru Komatsu
Satoshi Shimura
Tatsuya Nakamura
Tomomi Kofuku
Kaori Satoh
Isao Nishi
Hirofumi Toda
Hisaaki Nishio
Nozomi Fukuda
Hideo Watari
Tamotsu Ono
Tamaki Orita
Tetsu Mizutani
Machiko Kita
Keigo Kimura
Kaneyuki Kida
Saori Fukuda
Masanobu Ohama
Noriyuki Sueyoshi
Isako Nakai
Kana Sawa
Katsutoshi Yamasaki
Makoto Niki
Source :
Journal of Infection and Chemotherapy. 24:262-266
Publication Year :
2018
Publisher :
Elsevier BV, 2018.

Abstract

Carbapenemase-producing Enterobacteriaceae (CPE) are increasing worldwide. Rapid and accurate detection of CPE is necessary for appropriate antimicrobial treatment and hospital infection control. However, CPE contains some strains that are difficult to detect depending on genotype and MIC value of carbapenem, and a detection method has not been established. The recently reported modified carbapenem inactivation method (mCIM) has been developed in CLSI M100-S27 as a phenotypic technique for detecting carbapenemase activity. In the present study, we examined mCIM as a new CPE detection method using 207 Enterobacteriaceae isolates in comparison with the three existing screening methods of modified Hodge test, Carba NP test and carbapenem inactivation method and evaluated its performance. Consequently, both the sensitivity and specificity of mCIM were 100%, indicating better results than the conventional screening methods. The mCIM is a useful tool for microbiology laboratories due to its simplicity, clear criteria, cost-effectiveness and availability at any laboratory.

Details

ISSN :
1341321X
Volume :
24
Database :
OpenAIRE
Journal :
Journal of Infection and Chemotherapy
Accession number :
edsair.doi.dedup.....92f51ad9d833190018135518492dc8f6
Full Text :
https://doi.org/10.1016/j.jiac.2017.11.010