Back to Search
Start Over
Supramammillary glutamate neurons are a key node of the arousal system
- Source :
- Nature Communications, Vol 8, Iss 1, Pp 1-16 (2017), Nature Communications, Nature communications, vol 8, iss 1
- Publication Year :
- 2017
- Publisher :
- Nature Publishing Group, 2017.
-
Abstract
- Basic and clinical observations suggest that the caudal hypothalamus comprises a key node of the ascending arousal system, but the cell types underlying this are not fully understood. Here we report that glutamate-releasing neurons of the supramammillary region (SuMvglut2) produce sustained behavioral and EEG arousal when chemogenetically activated. This effect is nearly abolished following selective genetic disruption of glutamate release from SuMvglut2 neurons. Inhibition of SuMvglut2 neurons decreases and fragments wake, also suppressing theta and gamma frequency EEG activity. SuMvglut2 neurons include a subpopulation containing both glutamate and GABA (SuMvgat/vglut2) and another also expressing nitric oxide synthase (SuMNos1/Vglut2). Activation of SuMvgat/vglut2 neurons produces minimal wake and optogenetic stimulation of SuMvgat/vglut2 terminals elicits monosynaptic release of both glutamate and GABA onto dentate granule cells. Activation of SuMNos1/Vglut2 neurons potently drives wakefulness, whereas inhibition reduces REM sleep theta activity. These results identify SuMvglut2 neurons as a key node of the wake−sleep regulatory system.<br />Supramammillary nucleus (SuM) neurons have been studied in the context of REM sleep but their possible role in mediating wakefulness is not known. Here the authors elucidate the distinct functional contributions of three subpopulations in the SuM on electrographical and behavioral arousal in mice using genetically targeted approaches.
- Subjects :
- 0301 basic medicine
Male
Hypothalamus, Posterior
Vesicular Inhibitory Amino Acid Transport Proteins
General Physics and Astronomy
Stimulation
Nitric Oxide Synthase Type I
Transgenic
Mice
0302 clinical medicine
Theta Rhythm
lcsh:Science
Mice, Knockout
Neurons
Multidisciplinary
biology
Chemistry
Glutamate receptor
Nitric oxide synthase
Hypothalamus
Neurological
Wakefulness
Sleep Research
Arousal
Cell type
Knockout
Science
Glutamic Acid
Sleep, REM
Mice, Transgenic
Posterior
Optogenetics
General Biochemistry, Genetics and Molecular Biology
Article
03 medical and health sciences
Behavioral and Social Science
Animals
Neurosciences
General Chemistry
030104 developmental biology
nervous system
REM
biology.protein
Vesicular Glutamate Transport Protein 2
lcsh:Q
Sleep
Neuroscience
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 20411723
- Volume :
- 8
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Nature Communications
- Accession number :
- edsair.doi.dedup.....92f32695d3a5c9c57892887d7071348d