HISTOCHEMICAL DEMONSTRATION OF OXIDATIVE ENZYMES IN HUMAN SALIVARY GLANDS

Histochemical response have been determined on hnman three salivary glands using the Nitro BT method. Succinic dehydrogenase, DPN-dependent dehydrogenasee (malic, lactic, glutamic. α-glycerophosphate and β-hydroxybutyric), TPN-dependent dehydrogenase (isocitric, glucose-6-phosphate and aconitic), cytochrome oxidase and monoamine oxidase were demonstrated on cryostat sections.The intensity of staining reaction in the duct, especially in the intralobular duct or striated duct was the highest for succinic dehydrogenase and cytochrome oxidase, and other coenzyme dependent enzymes showed from a comparatively high to low reaction.The enzymatic activity of serous acini was more intense than that of mucous acini.